Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering

dc.contributor.authorForoutan Kalourazi, Ali
dc.contributor.authorNazemi, Seyed Amirabbas
dc.contributor.authorUnniram Parambil, Ajmal Roshan
dc.contributor.authorMuñoz-Tafalla, Ruben
dc.contributor.authorVidal, Paula
dc.contributor.authorShahangian, S. Shirin
dc.contributor.authorGuallar, Victor
dc.contributor.authorFerrer, Manuel
dc.contributor.authorShahgaldian, Patrick
dc.date.accessioned2025-03-10T14:33:56Z
dc.date.issued2024
dc.description.abstractWe report a method of enzyme stabilisation exploiting the artificial protein chaperone properties of β-cyclodextrin (β-CD) covalently embedded in an ultrathin organosilica layer. Putative interaction points of this artificial chaperone system with the surface of the selected enzyme were studied in silico using a protein energy landscape exploration simulation algorithm. We show that this enzyme shielding method allows for drastic enhancement of enzyme stability under thermal and chemical stress conditions, along with broadening the optimal temperature range of the biocatalyst. The presence of the β-CD macrocycle within the protective layer supports protein refolding after treatment with a surfactant.
dc.identifier.doi10.1039/d3nr06044f
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/49941
dc.identifier.urihttps://doi.org/10.26041/fhnw-11812
dc.issue10
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofNanoscale
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc500 - Naturwissenschaften und Mathematik
dc.titleExploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume16
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciences FHNWde_CH
fhnw.affiliation.institutInstitut für Chemie und Bioanalytikde_CH
fhnw.openAccessCategoryHybrid
fhnw.pagination5123-5129
fhnw.publicationStatePublished
relation.isAuthorOfPublication8884cd16-817b-4fba-a564-50a45970baa2
relation.isAuthorOfPublication.latestForDiscovery8884cd16-817b-4fba-a564-50a45970baa2
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