Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering
dc.contributor.author | Foroutan Kalourazi, Ali | |
dc.contributor.author | Nazemi, Seyed Amirabbas | |
dc.contributor.author | Unniram Parambil, Ajmal Roshan | |
dc.contributor.author | Muñoz-Tafalla, Ruben | |
dc.contributor.author | Vidal, Paula | |
dc.contributor.author | Shahangian, S. Shirin | |
dc.contributor.author | Guallar, Victor | |
dc.contributor.author | Ferrer, Manuel | |
dc.contributor.author | Shahgaldian, Patrick | |
dc.date.accessioned | 2025-03-10T14:33:56Z | |
dc.date.issued | 2024 | |
dc.description.abstract | We report a method of enzyme stabilisation exploiting the artificial protein chaperone properties of β-cyclodextrin (β-CD) covalently embedded in an ultrathin organosilica layer. Putative interaction points of this artificial chaperone system with the surface of the selected enzyme were studied in silico using a protein energy landscape exploration simulation algorithm. We show that this enzyme shielding method allows for drastic enhancement of enzyme stability under thermal and chemical stress conditions, along with broadening the optimal temperature range of the biocatalyst. The presence of the β-CD macrocycle within the protective layer supports protein refolding after treatment with a surfactant. | |
dc.identifier.doi | 10.1039/d3nr06044f | |
dc.identifier.issn | 2040-3364 | |
dc.identifier.issn | 2040-3372 | |
dc.identifier.uri | https://irf.fhnw.ch/handle/11654/49941 | |
dc.identifier.uri | https://doi.org/10.26041/fhnw-11812 | |
dc.issue | 10 | |
dc.language.iso | en | |
dc.publisher | Royal Society of Chemistry | |
dc.relation.ispartof | Nanoscale | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.ddc | 500 - Naturwissenschaften und Mathematik | |
dc.title | Exploiting cyclodextrins as artificial chaperones to enhance enzyme protection through supramolecular engineering | |
dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
dc.volume | 16 | |
dspace.entity.type | Publication | |
fhnw.InventedHere | Yes | |
fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
fhnw.affiliation.hochschule | Hochschule für Life Sciences FHNW | de_CH |
fhnw.affiliation.institut | Institut für Chemie und Bioanalytik | de_CH |
fhnw.openAccessCategory | Hybrid | |
fhnw.pagination | 5123-5129 | |
fhnw.publicationState | Published | |
relation.isAuthorOfPublication | 8884cd16-817b-4fba-a564-50a45970baa2 | |
relation.isAuthorOfPublication.latestForDiscovery | 8884cd16-817b-4fba-a564-50a45970baa2 |
Dateien
Originalbündel
1 - 1 von 1
Lizenzbündel
1 - 1 von 1
Lade...
- Name:
- license.txt
- Größe:
- 2.66 KB
- Format:
- Item-specific license agreed upon to submission
- Beschreibung: