Biotransformation of ritalinic acid by laccase in the presence of mediator TEMPO

dc.accessRightsAnonymous
dc.audienceScience
dc.contributor.authorKobakhidze, Aza
dc.contributor.authorElisashvili, Vladimir
dc.contributor.authorCorvini, Philippe
dc.contributor.authorCvancarova Småstuen, M.
dc.date.accessioned2019-01-25T08:26:46Z
dc.date.available2019-01-25T08:26:46Z
dc.date.issued2018-07
dc.description.abstractMethylphenidate is widely used as a medication for the treatment of attention deficit hyperactivity disorder (ADHD) in children. Less than 1% of methylphenidate is excreted unchanged in urine, while 80% of an oral dose is excreted as ritalinic acid (which is reportedly poorly degradable). This study aims to investigate the biotransformation of ritalinic acid by free and immobilized enzymes. The influence of various laccase mediators on biotransformation efficiency has been tested. Formation of the main transformation products has been monitored and their potential structures suggested. The effective transformation of ritalinic acid was observed only in the presence of 2,2,6,6-tetramethylpiperidine 1-oxyl mediator (TEMPO). The most effective enzyme was the laccase of T. versicolor 159. The main transformation product was an N-methyl derivative of ritalinic acid. Ritalinic acid was also reduced to aldehyde and alcohol, and a broad spectrum of intermediate complexes with oxoammonium ion of TEMPO were detected. This is the first time the biotransformation of ritalinic acid has been investigated in detail.
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2017.08.008
dc.identifier.issn1871-6784
dc.identifier.issn1876-4347
dc.identifier.urihttp://hdl.handle.net/11654/27321
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofNew Biotechnologyen_US
dc.subjectRitalinic acid
dc.subjectLaccase
dc.subjectBiotransformation
dc.subjectTEMPO
dc.subjectLC-MS
dc.titleBiotransformation of ritalinic acid by laccase in the presence of mediator TEMPO
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume53
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.IsStudentsWorkno
fhnw.PublishedSwitzerlandNo
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciences FHNWde_CH
fhnw.affiliation.institutInstitut für Ecopreneurshipde_CH
fhnw.pagination44-52
fhnw.publicationOnlineJa
fhnw.publicationStatePublished
relation.isAuthorOfPublicationb70a3a4f-d739-4ef3-84c8-cab8e28c05c7
relation.isAuthorOfPublication.latestForDiscoveryb70a3a4f-d739-4ef3-84c8-cab8e28c05c7
Dateien