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dc.contributor.authorJähne, EA
dc.contributor.authorSampath, C
dc.contributor.authorButterweck, Veronika
dc.contributor.authorHamburger, Matthias
dc.contributor.authorOufir, M
dc.date.accessioned2016-11-09T11:29:42Z
dc.date.available2016-11-09T11:29:42Z
dc.date.issued2016
dc.identifier.issn0731-7085
dc.identifier.doi10.1016/j.jpba.2016.05.018
dc.identifier.urihttp://hdl.handle.net/11654/23428
dc.description.abstractAn UPLC-MS/MS method for the quantification of indirubin in lithium heparinized rat plasma was developed and validated according to current international guidelines. Indirubin was extracted from rat plasma by using Waters Ostro™ pass-through sample preparation plates. The method was validated with a LLOQ of 5.00ng/mL and an ULOQ of 500ng/mL. The calibration curve was fitted by least-square quadratic regression, and a weighting factor of 1/X was applied. Recoveries of indirubin and I.S. were consistent and ≥75.5%. Stability studies demonstrated that indirubin was stable in lithium heparinized rat plasma for at least 3 freeze/thaw cycles, for 3h at RT, for 96h in the autosampler at 10°C, and for 84days when stored below -65°C. Preliminary pharmacokinetic (PK) data were obtained from Sprague Dawley rats after intravenous administration of indirubin (2mg/kg b.w.) and blood sampling up to 12h after injection. PK parameters were determined by non-compartmental analysis. Indirubin had a half-life (t1/2) of 35min, and a relatively high clearance (CL) of 2.71L/h/kg.
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/27281580
dc.language.isoen
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.accessRightsAnonymous
dc.titleDevelopment and full validation of an UPLC-MS/MS method for the quantification of the plant-derived alkaloid indirubin in rat plasma
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.volume128
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandNo
fhnw.pagination247-252
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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