FHNW Fachhochschule Nordwestschweiz
  • Startseite
  • Publikationen
  • Projekte
  • Studentische Arbeiten
  • de
  •  Login
Eintraganzeige 
  •   IRF Home
  • Hochschule für Life Sciences
  • Institut für Ecopreneurship
  • Eintraganzeige
  • Hochschule für Life Sciences
  • Institut für Ecopreneurship
  • Eintraganzeige
JavaScript is disabled for your browser. Some features of this site may not work without it.

Comparative effects of nodularin and microcystin-LR in zebrafish: 1. Uptake by organic anion transporting polypeptide Oatp1d1 (Slco1d1)

Autor/Autorin
Faltermann, Susanne
Prétôt, René
Pernthaler, Jakob
Fent, Karl
Datum
02.2016
Metadata
Zur Langanzeige
Type
01 - Zeitschriftenartikel, Journalartikel oder Magazin
Zusammenfassung
Microcystin-LR (MC-LR) and nodularin are hepatotoxins produced by several cyanobacterial species. Their toxicity is based on active cellular uptake and subsequent inhibition of protein phosphatases PP1/2A, leading to hyperphosphorylation and cell death. To date, uptake of MC-LR and nodularin in fish is poorly understood. Here, we investigated the role of the organic anion transporting polypeptide Oatp1d1 in zebrafish (drOatp1d1, Slco1d1) in cellular uptake in zebrafish. We stably transfected CHO and HEK293 cell lines expressing drOatp1d1. In both transfectants, uptake of MC-LR and nodularin was demonstrated by competitive inhibition of uptake with fluorescent substrate lucifer yellow. Direct uptake of MC-LR was demonstrated by immunostaining, and indirectly by the high cytotoxicity in stable transfectants. By means of a synthesized fluorescent labeled MC-LR derivative, direct uptake was further confirmed in HEK293 cells expressing drOatp1d1. Additionally, uptake and toxicity was investigated in the permanent zebrafish liver cell line ZFL. These cells had only a low relative abundance of drOatp1d1, drOatp2b1 and drOatp1f transcripts, which correlated with the lack of MC-LR induced cytotoxicity and transcriptional changes of genes indicative of endoplasmic reticulum stress, a known effect of this toxin. Our study demonstrates that drOatp1d1 functions as an uptake transporter for both MC-LR and nodularin in zebrafish.
URI
http://hdl.handle.net/11654/24044
DOI der Originalausgabe
https://doi.org/10.1016/j.aquatox.2015.11.016.
Übergeordnetes Werk
Aquatic Toxicology
Jahrgang
171
Seiten
69-76
Zitation

Stöbern

Gesamter BestandBereiche & SammlungenErscheinungsdatumAutoren/AutorinnenTitelThemenDiese SammlungErscheinungsdatumAutoren/AutorinnenTitelThemen

Mein Benutzerkonto

EinloggenRegistrieren
Erweiterter Export: CSVErweiterter Export: RISErweiterter Export: BibTeX

Kontakt

Fachhochschule Nordwestschweiz FHNW
Vizepräsidium Hochschulentwicklung
Bahnhofstrasse 6
5210 Windisch

E-Mail: irf@fhnw.ch

Über das IRF

Das IRF ist das digitale Repositorium der Fachhochschule Nordwestschweiz FHNW. Es enthält Publikationen, studentische Arbeiten und Projekte.

Links

IRF Handbuch
Liste der IRF Power User
Feedbackformular

www.fhnw.ch | Impressum | Datenschutz | Urheberrecht | IRF-Reglement