Determination of two progestin metabolites (17a-hydroxypregnanolone and pregnanediol) and different classes of steroids (androgens, estrogens, clorticosteroids, progestins) in rivers and wastewaters by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
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Autor:innen
Zhang, Kun
Autor:in (Körperschaft)
Publikationsdatum
2018
Typ der Arbeit
Studiengang
Sammlung
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Science of the Total Environment
Themenheft
DOI der Originalpublikation
Reihe / Serie
Reihennummer
Jahrgang / Band
610-611
Ausgabe / Nummer
Seiten / Dauer
1164-1172
Patentnummer
Verlag / Herausgebende Institution
Elsevier
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
A highly sensitive and robust method was developed for routine analysis of two progestin metabolites, 17α-hydroxypregnanolone (17OH-Δ5P) and pregnanediol (PD), and 31 other natural and synthetic steroids and related metabolites (estrogens, androgens, corticosteroids, progestins) in river water, as well as influents and effluents of municipal wastewater treatment plants (WWTP) using HPLC-MS/MS combined with solid-phase extraction. For the various matrixes considered, the optimized method showed satisfactory performance with recoveries of 70–120% for most of target steroids. The method detection limits (MDLs) ranged from 0.01 to 3 ng/L for river water, 0.02 to 10 ng/L for WWTP effluents, and 0.1 to 40 ng/L for influents with good linearity and reproducibility. The developed method was successfully applied for the analysis of steroids in rivers and WWTP influent and effluents. WWTP influents concentrations of 17OH-Δ5P and PD were 51–256 ng/L and up to 400 ng/L, respectively, along with androstenedione (concentration range: 38–220 ng/L), testosterone (11–26 ng/L), estrone (2.3–37 ng/L), 17β-estradiol (N.D.–8.7 ng/L), 17α-hydroxyprogesterone (N.D.–66 ng/L), medroxyprogesterone acetate (N.D.–5.3 ng/L), and progesterone (2.0–22 ng/L), while only androstenedione (ADD), estrone (E1), and estriol (E3) were detected in effluent with concentrations ranging up to 1.7 ng/L, 0.90 ng/L and 0.8 ng/L, respectively. In river water samples, only ADD and E1 were detected with concentrations up to 1.0 ng/L and 0.91 ng/L. Our procedure represents the first method for analyzing 17OH-Δ5P and PD in environmental samples along with a large series of steroids.
Schlagwörter
Steroids, Matrix effects, River water, Wastewater effluents, Trace analysis
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
0048-9697
1879-1026
1879-1026
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
FENT, Karl und Kun ZHANG, 2018. Determination of two progestin metabolites (17a-hydroxypregnanolone and pregnanediol) and different classes of steroids (androgens, estrogens, clorticosteroids, progestins) in rivers and wastewaters by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Science of the Total Environment. 2018. Bd. 610-611, S. 1164–1172. Verfügbar unter: http://hdl.handle.net/11654/25721