Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract
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Autor:innen
Corradi, Elisabetta
Schmidt, Nadine
Räber, Nathalie
De Mieri, Maria
Hamburger, Matthias
Potterat, Oliver
Butterweck, Veronika
Autor:in (Körperschaft)
Publikationsdatum
2017
Typ der Arbeit
Studiengang
Sammlung
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Ãœbergeordnetes Werk
Planta Medica
Themenheft
Link
Reihe / Serie
Jahrgang / Band
83
Ausgabe / Nummer
14/15
Seiten / Dauer
1149-1158
Patentnummer
Verlag / Herausgebende Institution
Thieme
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Phenolic constituents of Salix reticulata (Salicaceae) and antiproliferative activity of an extract and individual compounds were investigated in immortalized human non-tumorigenic keratinocytes (HaCaT). A MeOH extract from aerial parts afforded several flavonoids, including luteolin and apigenin glycosides (2–5 and 9) and catechin (1), two procyanidin fractions, and the phenolic glucosides picein (6), triandrin (7), and salicortin (8). In an adenosine triphosphate assay, the MeOH extract reduced cell viability by approximately 60 % at a concentration of 100 µg/mL. Cell proliferation was assessed with a BrdU incorporation ELISA assay. The extract inhibited proliferation of HaCaT cells in a concentration-dependent manner, with approximately 50 % inhibition at 100 µg/mL. In time-lapse assays, the extract showed distinct inhibitory effects on cell migration at concentrations of 12.5, 25, and 50 µg/mL. The activity of selected constituents was also determined. Luteolin-7-O-β-glucuronide (3) significantly inhibited cell proliferation at concentrations of 10 and 50 µM. In contrast, luteolin-7-O-β-glucopyranoside (2) and a procyanidin fraction (P1) had only weak effects, while picein (6) and salicortin (8) did not affect cell proliferation. Luteolin-7-O-β-glucuronide (10 µM) and, to a lesser extent, the procyanidin fraction (10 µg/mL) also inhibited cell migration.
Schlagwörter
Salicaceae, procyanidins, phenolic glucosides, luteolin-7-O-β-glucuronide, antiproliferative activity, cell migration, Salix reticulata
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
1439-0221
0032-0943
0032-0943
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Lizenz
Zitation
CORRADI, Elisabetta, Nadine SCHMIDT, Nathalie RÄBER, Maria DE MIERI, Matthias HAMBURGER, Oliver POTTERAT und Veronika BUTTERWECK, 2017. Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract. Planta Medica. 2017. Bd. 83, Nr. 14/15, S. 1149–1158. DOI 10.1055/s-0043-109098. Verfügbar unter: http://hdl.handle.net/11654/25794