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dc.contributor.authorCorradi, Elisabetta
dc.contributor.authorSchmidt, Nadine
dc.contributor.authorRäber, Nathalie
dc.contributor.authorDe Mieri, Maria
dc.contributor.authorHamburger, Matthias
dc.contributor.authorPotterat, Oliver
dc.contributor.authorButterweck, Veronika
dc.date.accessioned2018-01-10T09:25:20Z
dc.date.available2018-01-10T09:25:20Z
dc.date.issued2017
dc.identifier.doi10.1055/s-0043-109098
dc.identifier.urihttp://hdl.handle.net/11654/25794
dc.description.abstractPhenolic constituents of Salix reticulata (Salicaceae) and antiproliferative activity of an extract and individual compounds were investigated in immortalized human non-tumorigenic keratinocytes (HaCaT). A MeOH extract from aerial parts afforded several flavonoids, including luteolin and apigenin glycosides (2–5 and 9) and catechin (1), two procyanidin fractions, and the phenolic glucosides picein (6), triandrin (7), and salicortin (8). In an adenosine triphosphate assay, the MeOH extract reduced cell viability by approximately 60 % at a concentration of 100 µg/mL. Cell proliferation was assessed with a BrdU incorporation ELISA assay. The extract inhibited proliferation of HaCaT cells in a concentration-dependent manner, with approximately 50 % inhibition at 100 µg/mL. In time-lapse assays, the extract showed distinct inhibitory effects on cell migration at concentrations of 12.5, 25, and 50 µg/mL. The activity of selected constituents was also determined. Luteolin-7-O-β-glucuronide (3) significantly inhibited cell proliferation at concentrations of 10 and 50 µM. In contrast, luteolin-7-O-β-glucopyranoside (2) and a procyanidin fraction (P1) had only weak effects, while picein (6) and salicortin (8) did not affect cell proliferation. Luteolin-7-O-β-glucuronide (10 µM) and, to a lesser extent, the procyanidin fraction (10 µg/mL) also inhibited cell migration.
dc.language.isoen_US
dc.relation.ispartofPlanta Medica
dc.accessRightsAnonymous
dc.subjectSalicaceae
dc.subjectprocyanidins
dc.subjectphenolic glucosides
dc.subjectluteolin-7-O-β-glucuronide
dc.subjectantiproliferative activity
dc.subjectcell migration
dc.subjectSalix reticulata
dc.titleMetabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.volume83
dc.issue14/15
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandYes
fhnw.pagination1149-1158
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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