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dc.contributor.authorBoehringer, Stephan
dc.contributor.authorRuzgys, Paulius
dc.contributor.authorTamò, Luca
dc.contributor.authorSatkauskas, Saulius
dc.contributor.authorGeiser, Thomas
dc.contributor.authorGazdhar, Amiq
dc.contributor.authorHradetzky, David
dc.date.accessioned2018-12-13T09:53:47Z
dc.date.available2018-12-13T09:53:47Z
dc.date.issued2018-03
dc.identifier.doihttps://doi.org/10.1038/s41598-018-22280-2
dc.identifier.urihttp://hdl.handle.net/11654/26958
dc.description.abstractA challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.
dc.description.urihttps://www.nature.com/articles/s41598-018-22280-2#Abs1
dc.language.isoen
dc.relation.ispartofScientific Reports
dc.accessRightsAnonymous
dc.subjectelectrospray
dc.subjecttargeted gene delivery
dc.subjectcoulombs repulsion
dc.subjectElectric field
dc.titleA new electrospray method for targeted gene delivery
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.volume8
dc.issue1
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandNo
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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