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dc.contributor.authorWilli, Raffael Alois
dc.contributor.authorFaltermann, Susanne
dc.contributor.authorHettich, Timm
dc.contributor.authorFent, Karl
dc.date.accessioned2020-03-03T08:43:09Z
dc.date.available2020-03-03T08:43:09Z
dc.date.issued2019-07-01
dc.identifier.doi10.1016/j.scitotenv.2019.03.426.
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/30648
dc.description.abstractMany glucocorticoids occur in the aquatic environments but their adverse effects to fish are poorly known. Here we investigate effects of the natural glucocorticoid corticosterone and the synthetic glucocorticoids betamethasone and flumethasone in zebrafish embryos. Besides studying the effects of each steroid, we compared effects of natural with synthetic glucocorticoids, used as drugs. Exposure at concentrations of 1 μg/L and higher led to concentration-related decrease in spontaneous muscle contractions at 24 h post fertilization (hpf) and increase in heart rate at 48 hpf. Betamethasone showed a significant increase at 0.11 μg/L in heart rate. Corticosterone also accelerated hatching at 60 hpf at 0.085 μg/L. Transcription of up to 24 genes associated with different pathways showed alterations at 96 and 120 hpf for all glucocorticoids, although with low potency. Corticosterone caused transcriptional induction of interleukin-17, while betamethasone caused transcriptional down-regulation of the androgen receptor, aromatase and hsd11b2, indicating an effect on the sex hormone system. Furthermore, transcripts encoding proteins related to immune system regulation (irg1l, gilz) and fkbp5 were differentially expressed by corticosterone and betamethasone, while flumethasone caused only little effects, mainly alteration of the irg1l transcript. Our study shows that these glucocorticoids caused more potent physiological effects in early embryos than transcriptional alterations in hatched embryos, likely due to increased metabolism in later developmental stages. Thus, these glucocorticoids may be of concern for early stages of fish embryos in contaminated aquatic environments.en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pubmed/30954817en_US
dc.language.isoenen_US
dc.relation.ispartofScience of the Total Environmenten_US
dc.accessRightsAnonymous*
dc.subjectBetamethasoneen_US
dc.subjectCorticosteroneen_US
dc.subjectFlumethasoneen_US
dc.subjectGlucocorticoidsen_US
dc.subjectPhysiological effectsen_US
dc.subjectZebrafish embryosen_US
dc.titleEnvironmental glucocorticoids corticosterone, betamethasone and flumethasone induce more potent physiological than transcriptional effects in zebrafish embryos.en_US
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin*
dc.audienceScienceen_US
fhnw.publicationStatePublisheden_US
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publicationen_US
fhnw.InventedHereYesen_US
fhnw.PublishedSwitzerlandYesen_US
fhnw.pagination183-191en_US
fhnw.IsStudentsWorknoen_US
fhnw.publicationOnlineJaen_US


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