Kolvenbach, Boris
Lade...
E-Mail-Adresse
Geburtsdatum
Projekt
Organisationseinheiten
Berufsbeschreibung
Nachname
Kolvenbach
Vorname
Boris
Name
Kolvenbach, Boris
18 Ergebnisse
Suchergebnisse
Gerade angezeigt 1 - 10 von 18
Publikation Maize (Zea mays L.) plants change their fate and accumulate non-extractable residues of sulfamethoxazole in the soil of arable land(American Chemical Society, 16.05.2024) Wu, Xuan; Sun, Feifei; Cao, Siqi; Wang, Qilin; Wang, Lianhong; Wang, Songfeng; He, Yan; Kolvenbach, Boris; Corvini, Philippe; Ji, RongThe fate of sulfonamide antibiotics in farmlands is crucial for food and ecological safety, yet it remains unclear. We used [phenyl-U-14C]-labeled sulfamethoxazole (14C-SMX) to quantitatively investigate the fate of SMX in a soil–maize system for 60 days, based on a six-pool fate model. Formation of nonextractable residues (NERs) was the predominant fate for SMX in unplanted soil, accompanied by minor mineralization. Notably, maize plants significantly increased SMX dissipation (kinetic constant kd = 0.30 day–1 vs 0.17 day–1), while substantially reducing the NER formation (92% vs 58% of initially applied SMX) and accumulating SMX (40%, mostly bound to roots). Significant NERs (maximal 29–42%) were formed via physicochemical entrapment (determined using silylation), which could partially be released and taken up by maize plants. The NERs consisted of a considerable amount of SMX formed via entrapment (1–8%) and alkali-hydrolyzable covalent bonds (2–12%, possibly amide linkage). Six and 10 transformation products were quantified in soil extracts and NERs, respectively, including products of hydroxyl substitution, deamination, and N-acylation, among which N-lactylated SMX was found for the first time. Our findings reveal the composition and instability of SMX-derived NERs in the soil–plant system and underscore the need to study the long-term impacts of reversible NERs.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Investigation of biologically active zeolite. Role of colonization in the removal of 14C-labelled sulfamethoxazole in wastewater(Elsevier, 02/2024) Cuomo, Maurizio; König, Roger; Zanardini, Elisabetta; Di Guardo, Antonio; Terzaghi, Elisa; Kolvenbach, Boris; Demaria, Francesca; Corvini, Philippe; Principi, PamelaUp-to-date approaches to remove micropollutants in wastewater treatment are based on adsorbing materials like activated carbon. These fossil-based materials can also provide a surface for microbial colonization, which could further improve the removal of MPs. As zeolite filters have shown interesting performance in the removal of MPs in previous works, this study aimed to investigate the effect of microbial colonization on such filters on the elimination of 14C-labelled sulfamethoxazole (SMX), an antibiotic from the class of sulfonamides. Lab scale removal tests were set in 100 mL reactors and monitored for 150 days at room temperature. Taxa known to be linked to organic pollutant degradation (Caulobacterales, Rhizobiales, Burkholderiales) were found among the microbial community attached to the zeolite. Bacterial colonization of zeolite filters improved the removal of 14C-sulfamethoxazole by 35 % compared to the control. An analysis of the microbial community dynamics over time revealed the increased abundance of the Vicinamibacterales taxon after 50 days of contact with SMX. This order abundance, linked to degradation of sulfonamides, went from 0 to 17 %; and Shannon diversity ranged from 1.51 to 1.99. Data showed that zeolite filters as adsorbing material in wastewater treatment plants can improve MPs removal by supporting bacterial colonization, making it an interesting support that could synergize with biological activated carbon.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Pollutant profile complexity governs wastewater removal of recalcitrant pharmaceuticals(Nature, 2024) Suleiman, Marcel; Le Lay, Natalie; Demaria, Francesca; Kolvenbach, Boris; Cretoiu, Mariana; Petchey, Owen; Jousset, Alexandre; Corvini, PhilippeOrganic pollutants are an increasing threat for wildlife and humans. Managing their removal is however complicated by the difficulties in predicting degradation rates. In this work, we demonstrate that the complexity of the pollutant profile, the set of co-existing contaminants, is a major driver of biodegradation in wastewater. We built representative assemblages out of one to five common pharmaceuticals (caffeine, atenolol, paracetamol, ibuprofen, and enalapril) selected along a gradient of biodegradability. We followed their individual removal by wastewater microbial communities. The presence of multichemical background pollution was essential for the removal of recalcitrant molecules such as ibuprofen. High-order interactions between multiple pollutants drove removal efficiency. We explain these interactions by shifts in the microbiome, with degradable molecules such as paracetamol enriching species and pathways involved in the removal of several organic pollutants. We conclude that pollutants should be treated as part of a complex system, with emerging pollutants potentially showing cascading effects and offering leverage to promote bioremediation.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Analyzing microbial communities and their biodegradation of multiple pharmaceuticals in membrane bioreactors(Springer, 12.07.2023) Suleiman, Marcel; Demaria, Francesca; Zimmardi, Cristina; Kolvenbach, Boris; Corvini, PhilippeAbstract Pharmaceuticals are of concern to our planet and health as they can accumulate in the environment. The impact of these biologically active compounds on ecosystems is hard to predict, and information on their biodegradation is necessary to establish sound risk assessment. Microbial communities are promising candidates for the biodegradation of pharmaceuticals such as ibuprofen, but little is known yet about their degradation capacity of multiple micropollutants at higher concentrations (100 mg/L). In this work, microbial communities were cultivated in lab-scale membrane bioreactors (MBRs) exposed to increasing concentrations of a mixture of six micropollutants (ibuprofen, diclofenac, enalapril, caffeine, atenolol, paracetamol). Key players of biodegradation were identified using a combinatorial approach of 16S rRNA sequencing and analytics. Microbial community structure changed with increasing pharmaceutical intake (from 1 to 100 mg/L) and reached a steady-state during incubation for 7 weeks on 100 mg/L. HPLC analysis revealed a fluctuating but significant degradation (30–100%) of five pollutants (caffeine, paracetamol, ibuprofen, atenolol, enalapril) by an established and stable microbial community mainly composed of Achromobacter, Cupriavidus, Pseudomonas and Leucobacter. By using the microbial community from MBR1 as inoculum for further batch culture experiments on single micropollutants (400 mg/L substrate, respectively), different active microbial consortia were obtained for each single micropollutant. Microbial genera potentially responsible for degradation of the respective micropollutant were identified, i.e. Pseudomonas sp. and Sphingobacterium sp. for ibuprofen, caffeine and paracetamol, Sphingomonas sp. for atenolol and Klebsiella sp. for enalapril. Our study demonstrates the feasibility of cultivating stable microbial communities capable of degrading simultaneously a mixture of highly concentrated pharmaceuticals in lab-scale MBRs and the identification of microbial genera potentially responsible for the degradation of specific pollutants. Key points • Multiple pharmaceuticals were removed by stable microbial communities. • Microbial key players of five main pharmaceuticals were identified.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Insights into the applications of 3D bioprinting for bioremediation technologies(Elsevier, 2021) Ke, Zhuang; Obamwonyi, Osagie; Kolvenbach, Boris; Ji, Rong; Liu, Shuangjiang; Jiang, Jiandong; Corvini, PhilippeA plethora of organic pollutants such as pesticides, polycyclic and halogenated aromatic hydrocarbons, and emerging pollutants, such as flame retardants, is continuously being released into the environment. This poses a huge threat to the society in terms of environmental pollution, agricultural product quality, and general safety. Therefore, effective removal of organic pollutants from the environment has become an important challenge to be addressed. As a consequence of the recent and rapid developments in additive manufacturing, 3D bioprinting technology is playing an important role in the pharmaceutical industry. At the same time, an increasing number of microorganisms suitable for the production of biomaterials with complex structures and functions using 3D bioprinting technology, have been identified. This article briefly discusses the principles, advantages, and disadvantages of different 3D bioprinting technologies for pollutant removal. Furthermore, the feasibility and challenges of developing bioremediation technologies based on 3D bioprinting have also been discussed01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Biodeterioration affecting efficiency and lifetime of plastic-based photovoltaics(Elsevier, 16.09.2020) Schmidt, Felix; Lenz, Markus; Schaeffer, Andreas; Zimmermann, Yannick; Alves dos Reis Benatto, Gisele; Kolvenbach, Boris; Krebs, FrederikThe low environmental impact of electricity generation using solar cells crucially depends on high energy-conversion efficiencies, long lifetimes and a minimal energy and material demand during production. Emerging thin-film photovoltaics such as perovskites on plastic substrates could hold promise to fulfil all these requirements. Under real-world operating conditions photovoltaic operation is challenged by biological stressors, which have not been incorporated for evaluation in any test. Such stressors cause biodeterioration, which impairs diverse, apparently inert materials such as rock, glass and steel and therefore could significantly affect the function and stability of plastic-based solar cells. Given that different photovoltaic technologies commonly use similar materials, the biodeterioration mechanisms reviewed here may possibly affect the efficiency and lifetimes of several technologies, if they occur sufficiently fast (during the expected lifetime of photovoltaics). Once the physical integrity of uppermost module layers is challenged by biofilm growth microbially mediated dissolution and precipitation reactions of photovoltaic functional materials are very likely to occur. The biodeterioration of substrates and seals also represents emission points for the release of potentially harmful photovoltaic constituents to the environment01A - Beitrag in wissenschaftlicher ZeitschriftPublikation In-situ recovery of carboxylic acids from fermentation broths through membrane supported reactive extraction using membrane modules with improved stability(Elsevier, 15.06.2020) Gössi, Angelo; Burgener, Florian; Kohler, David; Urso, Alessandro; Kolvenbach, Boris; Riedl, WolfgangMembrane supported reactive extraction (MSE) coupled to back-extraction (MSBE) using a new type of Teflon (PTFE) capillary membrane contactor was studied for the in-situ removal of carboxylic acids from aqueous streams, e.g. fermentation broths. The use of microporous membranes as extraction interface helps avoiding emulsification problems, allows the use of extreme phase ratios, and protects microorganisms, as they are less affected by solvent toxicity during in-situ extractions. The use of PTFE capillary membranes is suitable for long-term use due its high chemical and thermal stability. A simple toxicity screening identified n-decanol with tri n-octyl amine (TOA) as a suitable solvent. MSE experiments were performed using membrane contactors (0.005 m2 to 0.15 m2), working with solvent to feed phase ratios down to 1:40 (mass based). The in-situ removal of lactic acid out of fermentation broths using lactobacillus plantarum led to a glucose conversion rate of 80 mol%. Additionally, a concentration factor up to 7.8 could be shown during back-extraction.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Biodegradation of sulfamethoxazole by a bacterial consortium of Achromobacter denitrificans PR1 and Leucobacter sp. GP(Springer, 12/2018) Reis, Ana C.; Cvancarova Småstuen, M.; Liu, Ying; Lenz, Markus; Hettich, Timm; Kolvenbach, Boris; Corvini, Philippe; Nunes, Olga C.In the last decade, biological degradation and mineralization of antibiotics have been increasingly reported feats of environmental bacteria. The most extensively described example is that of sulfonamides that can be degraded by several members of Actinobacteria and Proteobacteria. Previously, we reported sulfamethoxazole (SMX) degradation and partial mineralization by Achromobacter denitrificans strain PR1, isolated from activated sludge. However, further studies revealed an apparent instability of this metabolic trait in this strain. Here, we investigated this instability and describe the finding of a low-abundance and slow-growing actinobacterium, thriving only in co-culture with strain PR1. This organism, named GP, shared highest 16S rRNA gene sequence similarity (94.6–96.9%) with the type strains of validly described species of the genus Leucobacter. This microbial consortium was found to harbor a homolog to the sulfonamide monooxygenase gene (sadA) also found in other sulfonamide-degrading bacteria. This gene is overexpressed in the presence of the antibiotic, and evidence suggests that it codes for a group D flavin monooxygenase responsible for the ipso-hydroxylation of SMX. Additional side reactions were also detected comprising an NIH shift and a Baeyer–Villiger rearrangement, which indicate an inefficient biological transformation of these antibiotics in the environment. This work contributes to further our knowledge in the degradation of this ubiquitous micropollutant by environmental bacteria.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Arsenic Mobilization from Historically Contaminated Mining Soils in a Continuously Operated Bioreactor: Implications for Risk Assessment(Taylor & Francis, 2016) Rajpert, Liwia; Kolvenbach, Boris; Ammann, Erik; Hockmann, Kerstin; Nachtegaal, Maarten; Eiche, Elisabeth; Schäffer, Andreas; Corvini, Philippe; Sklodowska, Aleksandra; Lenz, MarkusConcentrations of soil arsenic (As) in the vicinity of the former Złoty Stok gold mine (Lower Silesia, southwest Poland) exceed 1000 μg g–1 in the area, posing an inherent threat to neighboring bodies of water. This study investigated continuous As mobilization under reducing conditions for more than 3 months. In particular, the capacity of autochthonic microflora that live on natural organic matter as the sole carbon/electron source for mobilizing As was assessed. A biphasic mobilization of As was observed. In the first two months, As mobilization was mainly conferred by Mn dissolution despite the prevalence of Fe (0.1 wt % vs 5.4 for Mn and Fe, respectively) as indicated by multiple regression analysis. Thereafter, the sudden increase in aqueous As[III] (up to 2400 μg L–1) was attributed to an almost quintupling of the autochthonic dissimilatory As-reducing community (quantitative polymerase chain reaction). The aqueous speciation influenced by microbial activity led to a reduction of solid phase As species (X-ray absorption fine structure spectroscopy) and a change in the elemental composition of As hotspots (micro X-ray fluorescence mapping). The depletion of most natural dissolved organic matter and the fact that an extensive mobilization of As[III] occurred after two months raises concerns about the long-term stability of historically As-contaminated sites.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Elucidating the genetic basis for sulfonamide degradation in isolates with diverse phylogenetic background(2015) Kolvenbach, Boris; Ricken, Benjamin; Corvini, Philippe; Reis, Ana C.; Nunes, Olga C.; Manaia, Célia M.; Strnad, Hynek; Vlcek, Cestmir06 - Präsentation