Auflistung nach Autor:in "Koehler, F. M."

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    Publikation
    β‐D‐glucosidase assisted gold dissolution as non‐optical and quantifiable detection technique for immunoassays
    (Wiley, 13.05.2013) Koehler, F. M.; Raso, Renzo; Grass, R. N.; Stark, W. J.
    Immunoassays are used for detecting protein targets for various applications. Here, a modification of immunoassays to allow a purely electrical detection of the target protein concentration is shown. The modification comprises a β-D-glucosidase as reporter enzyme and a cyanogenic glycoside as substrate. The enzymatic reaction produces cyanide in small quantities. For electrical detection of the cyanide, a novel sensor is developed, based on a gold micro wire. The cyanide dissolves the gold wire and changes the electrical resistance of the wire. Monitoring the resistance change allows a quantitative measurement of the target human C-reactive protein (an inflammatory marker) in blood plasma in the physiological relevant concentration range.
    01A - Beitrag in wissenschaftlicher Zeitschrift