Suter-Dick, Laura

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Suter-Dick
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Laura
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Suter-Dick, Laura

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20223
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Enddatum: 2023 × Thema: 600 - Technik, Medizin, angewandte Wissenschaften ×

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Gerade angezeigt 1 - 3 von 3
  • Vorschaubild
    Publikation
    Apical medium flowiInfluences the morphology and physiology of human proximal tubular cells in a microphysiological system
    (MDPI, 30.09.2022) Specioso, Gabriele; Bovard, David; Zanetti, Filippo; Maranzano, Fabio; Merg, Céline; Sandoz, Antonin; Titz, Bjoern; Dalcanale, Federico; Hoeng, Julia; Renggli, Kasper; Suter-Dick, Laura [in: Bioengineering]
    There is a lack of physiologically relevant in vitro human kidney models for disease modelling and detecting drug-induced effects given the limited choice of cells and difficulty implementing quasi-physiological culture conditions. We investigated the influence of fluid shear stress on primary human renal proximal tubule epithelial cells (RPTECs) cultured in the micro-physiological Vitrofluid device. This system houses cells seeded on semipermeable membranes and can be connected to a regulable pump that enables controlled, unidirectional flow. After 7 days in culture, RPTECs maintained physiological characteristics such as barrier integrity, protein uptake ability, and expression of specific transporters (e.g., aquaporin-1). Exposure to constant apical side flow did not cause cytotoxicity, cell detachment, or intracellular reactive oxygen species accumulation. However, unidirectional flow profoundly affected cell morphology and led to primary cilia lengthening and alignment in the flow direction. The dynamic conditions also reduced cell proliferation, altered plasma membrane leakiness, increased cytokine secretion, and repressed histone deacetylase 6 and kidney injury molecule 1 expression. Cells under flow also remained susceptible to colistin-induced toxicity. Collectively, the results suggest that dynamic culture conditions in the Vitrofluid system promote a more differentiated phenotype in primary human RPTECs and represent an improved in vitro kidney model.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild
    Publikation
    Directional submicrofiber hydrogel composite scaffolds supporting neuron differentiation and enabling neurite alignment
    (MDPI, 29.09.2022) Selvi, Jasmin; Faia-Torres, Ana Bela; Rühe, Jürgen; Züger, Fabian; Suter-Dick, Laura; Mungenast, Lena; Gullo, Maurizio [in: International Journal of Molecular Sciences]
    Cell cultures aiming at tissue regeneration benefit from scaffolds with physiologically relevant elastic moduli to optimally trigger cell attachment, proliferation and promote differentiation, guidance and tissue maturation. Complex scaffolds designed with guiding cues can mimic the anisotropic nature of neural tissues, such as spinal cord or brain, and recall the ability of human neural progenitor cells to differentiate and align. This work introduces a cost-efficient gelatin-based submicron patterned hydrogel–fiber composite with tuned stiffness, able to support cell attachment, differentiation and alignment of neurons derived from human progenitor cells. The enzymatically crosslinked gelatin-based hydrogels were generated with stiffnesses from 8 to 80 kPa, onto which poly(ε-caprolactone) (PCL) alignment cues were electrospun such that the fibers had a preferential alignment. The fiber–hydrogel composites with a modulus of about 20 kPa showed the strongest cell attachment and highest cell proliferation, rendering them an ideal differentiation support. Differentiated neurons aligned and bundled their neurites along the aligned PCL filaments, which is unique to this cell type on a fiber–hydrogel composite. This novel scaffold relies on robust and inexpensive technology and is suitable for neural tissue engineering where directional neuron alignment is required, such as in the spinal cord.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild
    Publikation
    3D printed microfluidic modules. Passive mixers and cells encapsulation in alginate
    (De Gruyter, 02.09.2022) Dalcanale, Federico; Caj, Michaela; Schuler, Felix; Ganeshanathan, Kireedan; Suter-Dick, Laura [in: Current Directions in Biomedical Engineering]
    Passive mixers and droplet generation microfluidic chip modules were designed and manufactured using a commercial SLA 3D-printer. The mixing modules were designed specifically for 3D-printing and evaluated using FEM modeling. The co-flow droplet generator was used for cancer cells encapsulation and drug potency evaluation.
    01A - Beitrag in wissenschaftlicher Zeitschrift