Co‐current filtrate flow in TFF perfusion processes. Decoupling transmembrane pressure from crossflow to improve product sieving

dc.contributor.authorRomann, Patrick
dc.contributor.authorGiller, Philip
dc.contributor.authorSibilia, Antony
dc.contributor.authorHerwig, Christoph
dc.contributor.authorZydney, Andrew L.
dc.contributor.authorPerilleux, Arnaud
dc.contributor.authorSouquet, Jonathan
dc.contributor.authorBielser, Jean‐Marc
dc.contributor.authorVilliger, Thomas
dc.date.accessioned2024-02-06T08:40:48Z
dc.date.available2024-02-06T08:40:48Z
dc.date.issued2023
dc.description.abstractHollow fiber‐based membrane filtration has emerged as the dominant technology for cell retention in perfusion processes yet significant challenges in alleviating filter fouling remain unsolved. In this work, the benefits of co‐current filtrate flow applied to a tangential flow filtration (TFF) module to reduce or even completely remove Starling recirculation caused by the axial pressure drop within the module was studied by pressure characterization experiments and perfusion cell culture runs. Additionally, a novel concept to achieve alternating Starling flow within unidirectional TFF was investigated. Pressure profiles demonstrated that precise flow control can be achieved with both lab‐scale and manufacturing‐scale filters. TFF systems with co‐current flow showed up to 40% higher product sieving compared to standard TFF. The decoupling of transmembrane pressure from crossflow velocity and filter characteristics in co‐current TFF alleviates common challenges for hollow fiber‐based systems such as limited crossflow rates and relatively short filter module lengths, both of which are currently used to avoid extensive pressure drop along the filtration module. Therefore, co‐current filtrate flow in unidirectional TFF systems represents an interesting and scalable alternative to standard TFF or alternating TFF operation with additional possibilities to control Starling recirculation flow.
dc.identifier.doi10.1002/bit.28589
dc.identifier.issn0006-3592
dc.identifier.issn1097-0290
dc.identifier.urihttps://irf.fhnw.ch/handle/11654/44018
dc.identifier.urihttps://doi.org/10.26041/fhnw-7916
dc.issue2
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology & Bioengineering
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectStarling recirculation
dc.subjectPerfusion cell culture
dc.subjectTangential flow filtration (TFF)
dc.subject.ddc600 - Technik, Medizin, angewandte Wissenschaften
dc.titleCo‐current filtrate flow in TFF perfusion processes. Decoupling transmembrane pressure from crossflow to improve product sieving
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume121
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciencesde_CH
fhnw.affiliation.institutInstitut für Pharma Technologyde_CH
fhnw.openAccessCategoryHybrid
fhnw.pagination640-654
fhnw.publicationStatePublished
relation.isAuthorOfPublication5dacfc5f-c485-4afb-a603-8a8dee82fd8e
relation.isAuthorOfPublication78aa6e48-3093-4a61-be3e-05a07b581ef1
relation.isAuthorOfPublication4d5a9fac-da70-4ce6-a880-3118827dcf19
relation.isAuthorOfPublication.latestForDiscovery5dacfc5f-c485-4afb-a603-8a8dee82fd8e
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