Immobilisation and stabilisation of glycosylated enzymes on boronic acid-functionalised silica nanoparticles

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Authors
Nazemi, Seyed
Olesinska, Magdalena
Pezzella, Cinzia
Varriale, Simona
Lin, Chia-Wei
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Publication date
2021
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01A - Journal article
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Chemical Communications
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Volume
57
Issue / Number
Pages / Duration
11960-11963
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Royal Society of Chemistry
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Abstract
We report a method of glycosylated enzymes’ surface immobilisation and stabilisation. The enzyme is immobilised at the surface of silica nanoparticles through the reversible covalent binding of vicinal diols of the enzyme glycans with a surface-attached boronate derivative. A soft organosilica layer of controlled thickness is grown at the silica surface, entrapping the enzyme and thus avoiding enzyme leaching. We demonstrate that this approach results not only in high and durable activity retention but also enzyme stabilisation.
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1359-7345
1364-548X
Language
English
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Yes
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Published
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Peer review of the complete publication
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Closed
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Citation
NAZEMI, Seyed, Magdalena OLESINSKA, Cinzia PEZZELLA, Simona VARRIALE, Chia-Wei LIN, Philippe CORVINI und Patrick SHAHGALDIAN, 2021. Immobilisation and stabilisation of glycosylated enzymes on boronic acid-functionalised silica nanoparticles. Chemical Communications. 2021. Bd. 57, S. 11960–11963. DOI 10.1039/d1cc04916j. Verfügbar unter: https://irf.fhnw.ch/handle/11654/33396