Novel microcalorimetric assay for antibacterial activity of implant coatings. The cases of silver‐doped hydroxyapatite and calcium hydroxide
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Authors
Braissant, Olivier
Chavanne, Philippe
Stevanovic, Sabrina
Straumann, Lukas
Wirz, Dieter
Gruner, Philipp
Bachmann, Alexander
Author (Corporation)
Publication date
08/2015
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Type
01A - Journal article
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Parent work
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Special issue
DOI of the original publication
Link
Series
Series number
Volume
103
Issue / Number
6
Pages / Duration
1161 - 1167
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Publisher / Publishing institution
Wiley
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Abstract
Biomaterials with antimicrobial properties are now commonly used in different clinical specialties including orthopedics, endodontic, and traumatology. As a result, assessing the antimicrobial effect of coatings applied on implants is of critical importance. In this study, we demonstrate that isothermal microcalorimetry (IMC) can be used for monitoring bacterial growth and biofilm formation at the surface of such coatings and for determining their antimicrobial effects. The antibacterial effects of silver doped hydroxyapatite (HA) and calcium hydroxide coatings on Staphylococcus epidermidis were determined with a minimal workload. Using the Gompertz growth model we determined biofilm growth rates close to those values reported in the literature. Furthermore, we were able to estimate the reduction in the bacterial inocula originally applied at the surface of the coatings. Therefore, in addition to monitoring the antimicrobial effect of silver doped HA and calcium hydroxide coatings, we also demonstrate that IMC might be a valuable tool for assessing such antimicrobial properties of implant coatings at a minimal workload.
Keywords
Antibacterial implant coatings, Calcium hydroxide, Hydroxyapatite, Isothermal microcalorimetry, Staphylococcus
Subject (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
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ISBN
ISSN
1552-4973
1552-4981
1552-4981
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
BRAISSANT, Olivier, Philippe CHAVANNE, Michael DE WILD, Uwe PIELES, Sabrina STEVANOVIC, Ralf SCHUMACHER, Lukas STRAUMANN, Dieter WIRZ, Philipp GRUNER, Alexander BACHMANN und Gernot BONKAT, 2015. Novel microcalorimetric assay for antibacterial activity of implant coatings. The cases of silver‐doped hydroxyapatite and calcium hydroxide. Journal of Biomedical Materials Research Part B: Applied Biomaterials. August 2015. Bd. 103, Nr. 6, S. 1161 – 1167. DOI 10.1002/jbm.b.33294. Verfügbar unter: https://irf.fhnw.ch/handle/11654/45841