Assessment of the electrolyte heterogeneity of tissues in mandibular bone-infiltrating head and neck cancer using laser-induced breakdown spectroscopy

Type
01A - Journal article
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Parent work
International Journal of Molecular Sciences
Special issue
DOI of the original publication
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Volume
25
Issue / Number
5
Pages / Duration
Patent number
Publisher / Publishing institution
MDPI
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Abstract
Laser-induced breakdown spectroscopy (LIBS) was recently introduced as a rapid bone analysis technique in bone-infiltrating head and neck cancers. Research efforts on laser surgery systems with controlled tissue feedback are currently limited to animal specimens and the use of nontumorous tissues. Accordingly, this study aimed to characterize the electrolyte composition of tissues in human mandibular bone-infiltrating head and neck cancer. Mandible cross-sections from 12 patients with bone-invasive head and neck cancers were natively investigated with LIBS. Representative LIBS spectra (n = 3049) of the inferior alveolar nerve, fibrosis, tumor stroma, and cell-rich tumor areas were acquired and histologically validated. Tissue-specific differences in the LIBS spectra were determined by receiver operating characteristics analysis and visualized by principal component analysis. The electrolyte emission values of calcium (Ca) and potassium (K) significantly (p < 0.0001) differed in fibrosis, nerve tissue, tumor stroma, and cell-rich tumor areas. Based on the intracellular detection of Ca and K, LIBS ensures the discrimination between the inferior alveolar nerve and cell-rich tumor tissue with a sensitivity of ≥95.2% and a specificity of ≥87.2%. The heterogeneity of electrolyte emission values within tumorous and nontumorous tissue areas enables LIBS-based tissue recognition in mandibular bone-infiltrating head and neck cancer.
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ISBN
ISSN
1422-0067
1661-6596
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
'https://creativecommons.org/licenses/by/4.0/'
Citation
Winnand, P., Börnsen, K. O., Ooms, M., Heitzer, M., Vohl, N., Lammert, M., Hölzle, F., & Modabber, A. (2024). Assessment of the electrolyte heterogeneity of tissues in mandibular bone-infiltrating head and neck cancer using laser-induced breakdown spectroscopy. International Journal of Molecular Sciences, 25(5). https://doi.org/10.3390/ijms25052607