Surface modification of nanofibrous matrices via layer-by-layer functionalized silk assembly for mitigating the foreign body reaction

dc.accessRightsAnonymous
dc.audienceScience
dc.contributor.authorQian, Yuna
dc.contributor.authorLi, Linhao
dc.contributor.authorSong, Yang
dc.contributor.authorDong, Lili
dc.contributor.authorChen, Peixing
dc.contributor.authorLi, Xiaoming
dc.contributor.authorCai, Kaiyong
dc.contributor.authorGermershaus, Oliver
dc.contributor.authorLi, Yang
dc.contributor.authorFan, Yubo
dc.date.accessioned2019-01-25T08:27:21Z
dc.date.available2019-01-25T08:27:21Z
dc.date.issued2018-05
dc.description.abstractThe inherent hydrophobicity and large surface area of electrospun synthetic polymeric scaffolds often cause non-specific protein adsorption, thereby influencing macrophage functions and eventually leading to fibrosis at the tissue-scaffold interface. This work reports fabrication of silk fibroin (SF)-functionalized electrospun polycaprolactone (PCL) fibers by single-component layer-by-layer assembly and decorate the SF with heparin disaccharide (HD), resulting in the non-covalent binding of interleukin-4 (IL-4) with the capacity to modulate macrophage polarization. A modified SF derivative was obtained by diazonium coupling and then covalently bonded with HD via click chemistry to eventually bind IL-4 efficiently and maintain its bioactivity. In vitro studies showed that IL-4 surface-functionalized nanofibrous scaffolds promoted polarization to M2 macrophages in the short-term. Importantly, in a murine subcutaneous implantation model, we found that promoting transient shifts in macrophage polarization at early stage can significantly inhibit the extent of the late foreign body reactions. Furthermore, the results of a transcriptomic profiling showed that MARK, PI3K, JNK and NF-κB signaling pathways played an important role in regulating the macrophage phenotypes in the SF/HD/IL-4-functionalized fibers. Our results suggest that such a strategy offers a new approach for utilizing biological agent surface functionalization to modulate the foreign body reaction to nanofibrous scaffolds.
dc.identifier.doi10.1016/j.biomaterials.2018.02.038
dc.identifier.issn0142-9612
dc.identifier.issn1878-5905
dc.identifier.urihttp://hdl.handle.net/11654/27325
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiomaterialsen_US
dc.subjectsilk fibroin
dc.subjectelectrospun
dc.subjectchick chemistry
dc.subjectmacrophage
dc.subjectforeign body reaction
dc.titleSurface modification of nanofibrous matrices via layer-by-layer functionalized silk assembly for mitigating the foreign body reaction
dc.type01A - Beitrag in wissenschaftlicher Zeitschrift
dc.volume164
dspace.entity.typePublication
fhnw.InventedHereYes
fhnw.IsStudentsWorkno
fhnw.PublishedSwitzerlandNo
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.affiliation.hochschuleHochschule für Life Sciencesde_CH
fhnw.affiliation.institutInstitut für Pharma Technologyde_CH
fhnw.pagination22-37
fhnw.publicationOnlineJa
fhnw.publicationStatePublished
relation.isAuthorOfPublication5f661671-2e22-4169-b24b-692dcfd2aed4
relation.isAuthorOfPublication.latestForDiscovery5f661671-2e22-4169-b24b-692dcfd2aed4
Dateien