Development of a unique rapid test to detect anti-bodies directed against an extended RBD of SARS-CoV-2 spike protein
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Authors
Brosi, Larissa
Bantleon, Frank
Melone, Anna
Überschlag, Marie-Eve
Dolce, Daniele
Giegelmann, Cedric
Gerspach, Michael
Dirscherl, Lorin
Author (Corporation)
Publication date
28.05.2021
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Chimia
Special issue
DOI of the original publication
Link
Series
Series number
Volume
75
Issue / Number
5
Pages / Duration
446-452
Patent number
Publisher / Publishing institution
Schweizerische Chemische Gesellschaft
Place of publication / Event location
Bern
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
Serological testing for antibodies directed against SARS-CoV-2 in patients may serve as a diagnostic tool to verify a previous infection and as surrogate for an elicited humoral immune response, ideally conferring immunity after infection or vaccination. Here, we present the recombinant expression of an extended receptor binding domain (RBD) of the SARS-CoV-2 Spike protein used as capture antigen in a unique rapid immunoassay to detect the presence of RBD binding antibodies with high sensitivity and specificity. As currently available vaccines focus on the Spike RBD as target, the developed test can also be used to monitor a successful immune response after vaccination with an RBD based vaccine.
Keywords
Antibodies, Lateral flow assay, Point-of-care, Rbd, Sars-cov-2
Subject (DDC)
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
2673-2424
0009-4293
0009-4293
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
BROSI, Larissa, Thomas VILLIGER, Frank BANTLEON, Anna MELONE, Marie-Eve ÜBERSCHLAG, Daniele DOLCE, Cedric GIEGELMANN, Michael GERSPACH, Lorin DIRSCHERL, Sherin PANIKULAM, Patrick ROMANN, Anna WESTON, Eric KÜBLER und Christian GERHOLD, 2021. Development of a unique rapid test to detect anti-bodies directed against an extended RBD of SARS-CoV-2 spike protein. Chimia. 28 Mai 2021. Bd. 75, Nr. 5, S. 446–452. DOI 10.2533/chimia.2021.446. Verfügbar unter: https://doi.org/10.26041/fhnw-4142