Microbial interactions drive the complete catabolism of the antibiotic sulfamethoxazole in activated sludge microbiomes
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Authors
Qi, Mengyuan
Liang, Bin
Ma, Xiaodan
Yan, Lei
Dong, Wenchen
Kong, Deyong
Zhang, Liying
Zhu, Haizhen
Gao, Shu-Hong
Author (Corporation)
Publication date
02.03.2021
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Environmental Science & Technology
Special issue
DOI of the original publication
Link
Series
Series number
Volume
55
Issue / Number
5
Pages / Duration
3270-3282
Patent number
Publisher / Publishing institution
American Chemical Society
Place of publication / Event location
Washington
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
Microbial communities are believed to outperform monocultures in the complete catabolism of organic pollutants via reduced metabolic burden and increased robustness to environmental challenges; however, the interaction mechanism in functional microbiomes remains poorly understood. Here, three functionally differentiated activated sludge microbiomes (S1: complete catabolism of sulfamethoxazole (SMX); S2: complete catabolism of the phenyl part of SMX ([phenyl]-SMX) with stable accumulation of its heterocyclic product 3-amino-5-methylisoxazole (3A5MI); A: complete catabolism of 3A5MI rather than [phenyl]-SMX) were enriched. Combining time-series cultivation-independent microbial community analysis, DNA-stable isotope probing, molecular ecological network analysis, and cultivation-dependent function verification, we identified key players involved in the SMX degradation process. Paenarthrobacter and Nocardioides were primary degraders for the initial cleavage of the sulfonamide functional group (-C-S-N- bond) and 3A5MI degradation, respectively. Complete catabolism of SMX was achieved by their cross-feeding. The co-culture of Nocardioides, Acidovorax, and Sphingobium demonstrated that the nondegraders Acidovorax and Sphingobium were involved in the enhancement of 3A5MI degradation. Moreover, we unraveled the internal labor division patterns and connections among the active members centered on the two primary degraders. Overall, the proposed methodology is promisingly applicable and would help generate mechanistic, predictive, and operational understanding of the collaborative biodegradation of various contaminants. This study provides useful information for synthetic activated sludge microbiomes with optimized environmental functions.
Keywords
Anti-Bacterial Agents, Biodegradation, Environmental, microbiota, sewage, Sulfamethoxazole, water pollutants
Subject (DDC)
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ISBN
ISSN
1520-5851
0013-936X
0013-936X
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
Corvini, P., Qi, M., Liang, B., Ma, X., Yan, L., Dong, W., Kong, D., Zhang, L., Zhu, H., Gao, S.-H., Jiang, J., Liu, S.-J., & Wang, A. (2021). Microbial interactions drive the complete catabolism of the antibiotic sulfamethoxazole in activated sludge microbiomes. Environmental Science & Technology, 55(5), 3270–3282. https://doi.org/10.1021/acs.est.0c06687