An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin
| dc.accessRights | Anonymous | |
| dc.audience | Science | |
| dc.contributor.author | Zhao, Jingming | |
| dc.contributor.author | Bachmann, Daniel G. | |
| dc.contributor.author | Lenz, Markus | |
| dc.contributor.author | Gillingham, Dennis G. | |
| dc.contributor.author | Ward, Thomas R. | |
| dc.date.accessioned | 2018-12-21T07:53:58Z | |
| dc.date.available | 2018-12-21T07:53:58Z | |
| dc.date.issued | 2018-04 | |
| dc.description.abstract | We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers). | |
| dc.identifier.doi | 10.1039/C8CY00646F | |
| dc.identifier.issn | 2044-4761 | |
| dc.identifier.issn | 2044-4753 | |
| dc.identifier.uri | http://hdl.handle.net/11654/27038 | |
| dc.issue | 8 | |
| dc.language.iso | en | |
| dc.publisher | Royal Society of Chemistry | en_US |
| dc.relation.ispartof | Catalysis Science & Technology | en_US |
| dc.subject | metalloenzymes | |
| dc.subject | biotinylated dirhodium core | |
| dc.subject | streptavidin | |
| dc.title | An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin | |
| dc.type | 01A - Beitrag in wissenschaftlicher Zeitschrift | |
| dc.volume | 2018 | |
| dspace.entity.type | Publication | |
| fhnw.InventedHere | Yes | |
| fhnw.IsStudentsWork | no | |
| fhnw.PublishedSwitzerland | No | |
| fhnw.ReviewType | Anonymous ex ante peer review of a complete publication | |
| fhnw.affiliation.hochschule | Hochschule für Life Sciences | de_CH |
| fhnw.affiliation.institut | Institut für Ecopreneurship | de_CH |
| fhnw.pagination | 2294-2298 | |
| fhnw.publicationOnline | Ja | |
| fhnw.publicationState | Published | |
| relation.isAuthorOfPublication | c7b0a617-ef2c-48b2-919e-18d2c62cc929 | |
| relation.isAuthorOfPublication.latestForDiscovery | c7b0a617-ef2c-48b2-919e-18d2c62cc929 |