Immobilization of an artificial imine reductase within silica nanoparticles improves its performance
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Authors
Hestericová, Martina
Ward, Thomas R.
Author (Corporation)
Publication date
2016
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Chemical Communications
Special issue
DOI of the original publication
Link
Series
Series number
Volume
52
Issue / Number
60
Pages / Duration
9462-9465
Patent number
Publisher / Publishing institution
Royal Society of Chemistry
Place of publication / Event location
Edition
Version
Programming language
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Practice partner / Client
Abstract
Silica nanoparticles equipped with an artificial imine reductase (biotinylated iridium complex conjugated with streptavidin) display marked redn. activity toward cyclic imines and NAD. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding >46,000 turnovers in pure samples and 4000 turnovers in crude cellular exts.
Keywords
Enzymes
Subject (DDC)
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ISBN
ISSN
1359-7345
1364-548X
1364-548X
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
Hestericová, M., Correro, M. R., Lenz, M., Corvini, P., Shahgaldian, P., & Ward, T. R. (2016). Immobilization of an artificial imine reductase within silica nanoparticles improves its performance. Chemical Communications, 52(60), 9462–9465. https://doi.org/10.1039/c6cc04604e