Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

Abstract

Silica nanoparticles equipped with an artificial imine reductase (biotinylated iridium complex conjugated with streptavidin) display marked redn. activity toward cyclic imines and NAD. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding >46,​000 turnovers in pure samples and 4000 turnovers in crude cellular exts.