Design of miniaturized electro spray instrument for gene and drug therapeutic treatment of IPF

Abstract

Electric field accelerated plasmid droplets to permeate tissues has been demonstrated on cell culture and ex-vivo lung tissue of rats using enhanced green fluorescence protein reporter gene for transfection. This opens a window of opportunity to design an electrospray instrument usable within the working channel of bronchoscope to noninvasively treat idiopathic pulmonary fibrosis (IPF). We elaborate a concept and realized a device usable in the working channel of a flexible bronchoscope taking into account restrictions within typical bronchoscopy procedure.