Comparison of plasma ionization- and secondary electrospray ionization- high-resolution mass spectrometry for real-time breath analysis
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Authors
Zeng, Jiafa
Christen, Alexandra
Dev Singh, Kapil
Frey, Urs
Sinues, Pablo
Author (Corporation)
Publication date
23.02.2022
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Chimia
Special issue
DOI of the original publication
Link
Series
Series number
Volume
76
Issue / Number
1-2
Pages / Duration
Patent number
Publisher / Publishing institution
Schweizerische Chemische Gesellschaft
Place of publication / Event location
Bern
Edition
Version
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Practice partner / Client
Abstract
Real-time breath analysis by high-resolution mass spectrometry (HRMS) is a promising method to noninvasively retrieve relevant biochemical information. In this work, we conducted a head-to-head comparison of two ionization techniques: Secondary electrospray ionization (SESI) and plasma ionization (PI), for the analysis of exhaled breath. Two commercially available SESI and PI sources were coupled to the same HRMS device to analyze breath of two healthy individuals in a longitudinal study. We analyzed 58 breath specimens in both platforms, leading to 2,209 and 2,296 features detected by SESI-HRMS and by PI-HRMS, respectively. 60% of all the mass spectral features were detected in both platforms. However, remarkable differences were noted in terms of the signal-to-noise ratio (S/N), whereby the median (interquartile range, IQR) S/N ratio for SESI-HRMS was 115 (IQR = 408), whereas for PI-HRMS it was 5 (IQR = 5). Differences in the mass spectral profiles for the same samples make the inter-comparability of both techniques problematic. Overall, we conclude that both techniques are excellent for real-time breath analysis because of the very rich mass spectral fingerprints. However, further work is needed to fully understand the exact metabolic insights one can gather using each of these platforms.
Keywords
Breath analysis, Mass spectrometry, Plasma ionization, secondary electrospray ionization
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ISBN
ISSN
2673-2424
0009-4293
0009-4293
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
Zeng, J., Christen, A., Dev Singh, K., Frey, U., & Sinues, P. (2022). Comparison of plasma ionization- and secondary electrospray ionization- high-resolution mass spectrometry for real-time breath analysis. Chimia, 76(1-2). https://doi.org/10.2533/chimia.2022.127