Comparison of plasma ionization- and secondary electrospray ionization- high-resolution mass spectrometry for real-time breath analysis
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Dateien
Autor:innen
Zeng, Jiafa
Christen, Alexandra
Dev Singh, Kapil
Frey, Urs
Sinues, Pablo
Autor:in (Körperschaft)
Publikationsdatum
23.02.2022
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Chimia
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
76
Ausgabe / Nummer
1-2
Seiten / Dauer
Patentnummer
Verlag / Herausgebende Institution
Schweizerische Chemische Gesellschaft
Verlagsort / Veranstaltungsort
Bern
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Real-time breath analysis by high-resolution mass spectrometry (HRMS) is a promising method to noninvasively retrieve relevant biochemical information. In this work, we conducted a head-to-head comparison of two ionization techniques: Secondary electrospray ionization (SESI) and plasma ionization (PI), for the analysis of exhaled breath. Two commercially available SESI and PI sources were coupled to the same HRMS device to analyze breath of two healthy individuals in a longitudinal study. We analyzed 58 breath specimens in both platforms, leading to 2,209 and 2,296 features detected by SESI-HRMS and by PI-HRMS, respectively. 60% of all the mass spectral features were detected in both platforms. However, remarkable differences were noted in terms of the signal-to-noise ratio (S/N), whereby the median (interquartile range, IQR) S/N ratio for SESI-HRMS was 115 (IQR = 408), whereas for PI-HRMS it was 5 (IQR = 5). Differences in the mass spectral profiles for the same samples make the inter-comparability of both techniques problematic. Overall, we conclude that both techniques are excellent for real-time breath analysis because of the very rich mass spectral fingerprints. However, further work is needed to fully understand the exact metabolic insights one can gather using each of these platforms.
Schlagwörter
Breath analysis, Mass spectrometry, Plasma ionization, secondary electrospray ionization
Fachgebiet (DDC)
500 - Naturwissenschaften
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
2673-2424
0009-4293
0009-4293
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Gold
Lizenz
Zitation
ZENG, Jiafa, Alexandra CHRISTEN, Kapil DEV SINGH, Urs FREY und Pablo SINUES, 2022. Comparison of plasma ionization- and secondary electrospray ionization- high-resolution mass spectrometry for real-time breath analysis. Chimia. 23 Februar 2022. Bd. 76, Nr. 1-2. DOI 10.2533/chimia.2022.127. Verfügbar unter: https://doi.org/10.26041/fhnw-4325