Expanding the potential of the solvent-assisted method to create bio-interfaces from amphiphilic block copolymers
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Autor:innen
Di Leone, Stefano
Vallapurackal, Jaicy
Yorulmaz Avsar, Saziye
Kyropolou, Myrto
Ward, Thomas
Palivan, Cornelia
Meier, Wolfgang
Autor:in (Körperschaft)
Publikationsdatum
09.06.2021
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Ãœbergeordnetes Werk
Biomacromolecules
Themenheft
Link
Reihe / Serie
Jahrgang / Band
22
Ausgabe / Nummer
7
Seiten / Dauer
3005-3016
Patentnummer
Verlag / Herausgebende Institution
American Chemical Society
Verlagsort / Veranstaltungsort
Washington
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Artificial membranes, as materials with biomimetic properties, can be applied in various fields, such as drug screening or bio-sensing. The solvent-assisted method (SA) represents a straightforward method to prepare lipid solid-supported membranes. It overcomes the main limitations of established membrane preparation methods, such as Langmuir–Blodgett (LB) or vesicle fusion. However, it has not yet been applied to create artificial membranes based on amphiphilic block copolymers, despite their enhanced mechanical stability compared to lipid-based membranes and bio-compatible properties. Here, we applied the SA method on different amphiphilic di- and triblock poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) copolymers and optimized the conditions to prepare artificial membranes on a solid support. The real-time membrane formation, the morphology, and the mechanical properties have been evaluated by a combination of atomic force microscopy and quartz crystal microbalance. Then, selected biomolecules including complementary DNA strands and an artificial deallylase metalloenzyme (ADAse) were incorporated into these membranes relying on the biotin–streptavidin technology. DNA strands served to establish the capability of these synthetic membranes to interact with biomolecules by preserving their correct conformation. The catalytic activity of the ADAse following its membrane anchoring induced the functionality of the biomimetic platform. Polymer membranes on solid support as prepared by the SA method open new opportunities for the creation of artificial membranes with tailored biomimetic properties and functionality.
Schlagwörter
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
1525-7797
1526-4602
1526-4602
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Closed
Lizenz
Zitation
DI LEONE, Stefano, Jaicy VALLAPURACKAL, Saziye YORULMAZ AVSAR, Myrto KYROPOLOU, Thomas WARD, Cornelia PALIVAN und Wolfgang MEIER, 2021. Expanding the potential of the solvent-assisted method to create bio-interfaces from amphiphilic block copolymers. Biomacromolecules. 9 Juni 2021. Bd. 22, Nr. 7, S. 3005–3016. DOI 10.1021/acs.biomac.1c00424. Verfügbar unter: https://irf.fhnw.ch/handle/11654/33389