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Expanding the potential of the solvent-assisted method to create bio-interfaces from amphiphilic block copolymers

Autor/Autorin
Di Leone, Stefano
Vallapurackal, Jaicy
Yorulmaz Avsar, Saziye
Kyropolou, Myrto
Ward, Thomas
Palivan, Cornelia
Meier, Wolfgang
Datum
09.06.2021
Metadata
Zur Langanzeige
Type
01 - Zeitschriftenartikel, Journalartikel oder Magazin
Zusammenfassung
Artificial membranes, as materials with biomimetic properties, can be applied in various fields, such as drug screening or bio-sensing. The solvent-assisted method (SA) represents a straightforward method to prepare lipid solid-supported membranes. It overcomes the main limitations of established membrane preparation methods, such as Langmuir–Blodgett (LB) or vesicle fusion. However, it has not yet been applied to create artificial membranes based on amphiphilic block copolymers, despite their enhanced mechanical stability compared to lipid-based membranes and bio-compatible properties. Here, we applied the SA method on different amphiphilic di- and triblock poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) copolymers and optimized the conditions to prepare artificial membranes on a solid support. The real-time membrane formation, the morphology, and the mechanical properties have been evaluated by a combination of atomic force microscopy and quartz crystal microbalance. Then, selected biomolecules including complementary DNA strands and an artificial deallylase metalloenzyme (ADAse) were incorporated into these membranes relying on the biotin–streptavidin technology. DNA strands served to establish the capability of these synthetic membranes to interact with biomolecules by preserving their correct conformation. The catalytic activity of the ADAse following its membrane anchoring induced the functionality of the biomimetic platform. Polymer membranes on solid support as prepared by the SA method open new opportunities for the creation of artificial membranes with tailored biomimetic properties and functionality.
URI
https://irf.fhnw.ch/handle/11654/33389
DOI der Originalausgabe
https://doi.org/10.1021/acs.biomac.1c00424
Übergeordnetes Werk
Biomacromolecules
Jahrgang
22
Ausgabe
7
Seiten
3005-3016
Verlag / Hrsg. Institution
American Chemical Society
Verlagsort / Veranstaltungsort
Washington
Zitation

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