Immobilisation and stabilisation of glycosylated enzymes on boronic acid-functionalised silica nanoparticles
Type
01 - Zeitschriftenartikel, Journalartikel oder Magazin
Zusammenfassung
We report a method of glycosylated enzymes’ surface immobilisation and stabilisation. The enzyme is immobilised at the surface of silica nanoparticles through the reversible covalent binding of vicinal diols of the enzyme glycans with a surface-attached boronate derivative. A soft organosilica layer of controlled thickness is grown at the silica surface, entrapping the enzyme and thus avoiding enzyme leaching. We demonstrate that this approach results not only in high and durable activity retention but also enzyme stabilisation.
DOI der Originalausgabe
https://doi.org/10.1039/d1cc04916jÜbergeordnetes Werk
Chemical Communications
Jahrgang
57
Seiten
11960-11963
Verlag / Hrsg. Institution
Royal Society of Chemistry