Perfluorocarbon-loaded poly(lactide-co-glycolide) nanoparticles from core to crust: Multifaceted Impact of Surfactant on Particle Ultrastructure, Stiffness, and Cell Uptake
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Autor:innen
Autor:in (Körperschaft)
Publikationsdatum
03.03.2025
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
ACS Applied Polymer Materials
Themenheft
DOI der Originalpublikation
Link
Reihe / Serie
Reihennummer
Jahrgang / Band
7
Ausgabe / Nummer
5
Seiten / Dauer
Patentnummer
Verlag / Herausgebende Institution
American Chemical Society
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Poly(lactide-co-glycolide) nanoparticles (PLGA NPs) loaded with Perfluoro-15-crown-5-ether (PFCE) have been developed for imaging applications. A slight modification of the formulation led to the formation of two distinct particle ultrastructures: multicore particles (MCPs) and core–shell particles (CSPs), where poly(vinyl alcohol) (PVA), a nonionic surfactant, and sodium cholate (NaCh), an anionic surfactant, were used, respectively. Despite their similar composition and colloidal characteristics, these particles have previously demonstrated significant differences in their in vivo distribution and clearance. We hypothesize that these differences are collectively driven by variations in their structural, chemical, and mechanical properties, which are investigated in this study. Nanomechanical characterizations of MCPs and CSPs by atomic force microscopy (AFM) revealed elastic modulus values of 54 and 270 MPa in water, respectively, indicating a better permeability and deformability of the multicore ultrastructure. The impact of the surfactant on the NP surface chemistry was evidenced by their protein corona, which was significantly greater in the CSPs. Additionally, an important amount of residual NaCh was found on the surface of CSPs, which formed strong interactions with bovine serum albumin (BSA), accounting for the difference in protein coronas and surface chemistry. Surprisingly, in vitro cell uptake studies showed a higher uptake of MCPs by RAW macrophages but a preference for CSPs by HeLa cells. We conclude that for this specific formulation and in this stiffness range, mechanical differences have a stronger impact in HeLa cells, while surface properties and chemical recognition play a more important role in uptake by macrophages. Overall, the extent to which a physical factor impacts cell uptake is highly dependent on the specific uptake mechanism. With this study, we provide an integrated perspective on the role of different surfactants in the particle formation process, their impact on particle ultrastructure, mechanical properties, and surface chemistry, and the overall effect on cell uptake in vitro.
Schlagwörter
Nanoparticles, AFM, Elastic modulus, PLGA, Ultrastructure, Cell uptake, Protein corona
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
2637-6105
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Zukunftsfelder FHNW
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Hybrid
Lizenz
Zitation
Vicente, N. L., Srinivas, M., & Tagit, O. (2025). Perfluorocarbon-loaded poly(lactide-co-glycolide) nanoparticles from core to crust: Multifaceted Impact of Surfactant on Particle Ultrastructure, Stiffness, and Cell Uptake. ACS Applied Polymer Materials, 7(5). https://doi.org/10.1021/acsapm.4c03360