Metabolomic serum abnormalities in dogs with hepatopathies
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Autor:innen
Imbery, Carolin A.
Dieterle, Frank
Ottka, Claudia
Weber, Corinna
Müller, Elisabeth
Lohi, Hannes
Giger, Urs
Autor:in (Körperschaft)
Publikationsdatum
29.03.2022
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Scientific Reports
Themenheft
DOI der Originalpublikation
Reihe / Serie
Reihennummer
Jahrgang / Band
12
Ausgabe / Nummer
1
Seiten / Dauer
Patentnummer
Verlag / Herausgebende Institution
Nature
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
Hepatopathies can cause major metabolic abnormalities in humans and animals. This study examined differences in serum metabolomic parameters and patterns in left-over serum samples from dogs with either congenital portosystemic shunts (cPSS, n = 24) or high serum liver enzyme activities (HLEA, n = 25) compared to control dogs (n = 64). A validated targeted proton nuclear magnetic resonance spectroscopy platform was used to assess 123 parameters. Principal component analysis of the serum metabolome demonstrated distinct clustering among individuals in each group, with the cluster of HLEA being broader compared to the other groups, presumably due to the wider spectrum of hepatic diseases represented in these samples. While younger and older adult control dogs had very similar metabolomic patterns and clusters, there were changes in many metabolites in the hepatopathy groups. Higher phenylalanine and tyrosine concentrations, lower branched-chained amino acids (BCAAs) concentrations, and altered fatty acid parameters were seen in cPSS dogs compared to controls. In contrast, dogs with HLEA had increased concentrations of BCAAs, phenylalanine, and various lipoproteins. Machine learning based solely on the metabolomics data showed excellent group classification, potentially identifying a novel tool to differentiate hepatopathies. The observed changes in metabolic parameters could provide invaluable insight into the pathophysiology, diagnosis, and prognosis of hepatopathies.
Schlagwörter
Fachgebiet (DDC)
500 - Naturwissenschaften
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
2045-2322
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Gold
Lizenz
Zitation
IMBERY, Carolin A., Frank DIETERLE, Claudia OTTKA, Corinna WEBER, Elisabeth MÜLLER, Hannes LOHI, Urs GIGER und Götz SCHLOTTERBECK, 2022. Metabolomic serum abnormalities in dogs with hepatopathies. Scientific Reports. 29 März 2022. Bd. 12, Nr. 1. DOI 10.1038/s41598-022-09056-5. Verfügbar unter: https://doi.org/10.26041/fhnw-4364