Hochschule für Life Sciences FHNW

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A method and tool for strategic hospital planning
(Springer, 2015) Brodbeck, Dominique; Degen, Markus; Walter, Andreas; Reichlin, Serge; Napierala, Christoph; Fred, Ana; Gamboa, Hugo; Elias, Dirk [in: Biomedical Engineering Systems and Technologies. 8th International Joint Conference, BIOSTEC 2015, Lisbon, Portugal, January 12-15, 2015, Revised Selected Papers]
We developed a visualization tool and a methodology to support strategic planning of hospital service portfolios. Hospitals in Switzerland are reimbursed with a fixed fee per case. The fixed-fee model makes medical services comparable from a financial point of view. In order to take advantage of this model, the data that characterizes the medical services must be operationalized. The method that we developed, centers around a visual metaphor that provides the basis for strategic thinking. It is complemented by a visualization tool that allows visualization, analysis, and modification of service portfolios. Special features enable the tool to be used during live planning sessions. We describe the method, the tool, and its application in strategy workshops for infrastructure planning, reorganization, and resource optimization decisions.
04B - Beitrag Konferenzschrift
A mobile collaboration and decision support system for the medical emergency departement
(SciTePress, 2012) Brodbeck, Dominique; Degen, Markus; Reiss, Maximilian; Conchon, Emmanuel; Correia, Carlos; Fred, Ana; Gamboa, Hugo [in: Proceedings of the International Conference on Health Informatics (HEALTHINF-2012)]
A hospital emergency department is a complex work environment, where the availability of the right information at the right time is crucial for efficient and safe operation. The current technology in use for communication and information management is mostly based on telephones and stationary personal computers. Modern smartphones with their computational power, voice, image, and video capabilities have the potential to play a significant role in improving the flow of information in the emergency department. We developed a system that explicitly supports the work flows of an emergency department. In addition to mobile access to patient data and notifications about the availability of diagnostic findings, it provides the possibility to supply media captured on-site to the patient record, and directly supports the consultation process.
04B - Beitrag Konferenzschrift
A novel concept combining experimental and mathematical analysis for the identification of unknown interspecies effects in a mixed culture
(Wiley, 08/2011) Schmidt, Julia K.; Riedele, Christian; Regestein, Lars; Rausenberger, Julia; Reichl, Udo [in: Biotechnology & Bioengineering]
Bacteria in natural habitats only occur in consortia together with various other species. Characterization of bacterial species, however, is normally done by laboratory testing of pure isolates. Any interactions that might appear during growth in mixed-culture are obviously missed by this approach. Existing experimental studies mainly focus on two-species mixed cultures with species specifically chosen for their known growth characteristics, and their anticipated interactions. Various theoretical mathematical studies dealing with mixed cultures and possible interspecies effects exist, but often models cannot be validated due to a lack of experimental data. Here, we present a concept for the identification of interspecies effects in mixed cultures with arbitrary and unknown single-species properties. Model structure and parameters were inferred from single-species experiments for the reproduction of mixed-culture experiments by simulation. A mixed culture consisting of the three-species Pseudomonas aeruginosa, Burkholderia cepacia, and Staphylococcus aureus served as a model system. For species-specific enumeration a quantitative terminal restriction length polymorphism (qT-RFLP) assay was used. Based on models fitted to single-species cultivations, the outcome of mixed-culture experiments was predicted. Deviations of simulation results and experimental findings were then used to design additional single-cell experiments, to modify the corresponding growth kinetics, and to update model parameters. Eventually, the resulting mixed-culture dynamics was predicted and compared again to experimental results. During this iterative cycle, it became evident that the observed coexistence of P. aeruginosa and B. cepacia in mixed-culture chemostat experiments cannot be explained on the basis of glucose as the only substrate. After extension of growth kinetics, that is, for use of amino acids as secondary substrates, mixed-culture simulations represented the experimental findings very well. According to the model structure, as motivated by single-species experiments, the growth of P. aeruginosa and B. cepacia on glucose and amino acids could be assumed to be independent of each other. In contrast, both substrates are taken up simultaneously by S. aureus.
01A - Beitrag in wissenschaftlicher Zeitschrift
A perfused in vitro human iPSC-derived blood–brain barrier faithfully mimics transferrin receptor-mediated transcytosis of therapeutic antibodies
(Springer, 2023) Burgio, Floriana; Gaiser, Carine; Brady, Kevin; Gatta, Viviana; Class, Reiner; Schrage, Ramona; Suter-Dick, Laura [in: Cellular and Molecular Neurobiology]
Delivering biologics to elicit a therapeutic response in the central nervous system (CNS) remains challenging due to the presence of the blood brain barrier (BBB). Receptor-mediated transcytosis is a strategy to improve brain exposure after systemic drug administration. The availability of a clinically relevant in vitro BBB model is crucial to investigate transcytosis pathways and to predict the penetration of biologics into the CNS. We created a perfused human in vitro BBB model made of induced pluripotent stem cells (iPSC)-derived brain microvascular endothelial cells (BMEC) for studying transferrin receptor-mediated transcytosis. iPSC-derived BMEC were seeded in the top channel of a three-lane microfluidic device (OrganoPlate®). After 2 days in culture, the established cell model exhibited relevant BBB features, including physiological transendothelial electrical resistance in a transwell setting (1500 Ω*cm), reduced apparent permeability (Papp) to the fluorescence tracer Lucifer yellow (20-fold less than cell-free chips), expression of key BBB markers such as tight junctions proteins, transporters, receptors and functional P-gp efflux pump. Moreover, the model exhibited functional transferrin receptor-mediated uptake and transcytosis. To assess selective transferrin receptor-mediated transcytosis, a mixture of anti-human transferrin receptor (MEM-189) and control (sheep IgG anti-bovine serum albumin) antibodies was perfused in the top channel for 2 h. The Papp of MEM-189 was 11-fold higher than that of the control antibody, demonstrating facilitated receptor-mediated transcytosis. Compared to published work reporting a 2-fold ratio, this result is remarkable and establishes the suitability of our model for exploring receptor-mediated transcytosis and screening of antibodies for putative brain shuttle application. A perfused in vitro human model made of iPSC-derived BMEC with the chief characteristics (barrier tightness, functionality) of the human BBB can be applied to study transferrin receptor (TfR)-mediated transcytosis of therapeutic antibodies. This may bring critical advances in drug shuttle technology. Graphical abstract generated with biorender.com.
01A - Beitrag in wissenschaftlicher Zeitschrift
An integrative model for phytochrome B mediated photomorphogenesis. From protein dynamics to physiology
(Public Library of Science, 19.05.2010) Rausenberger, Julia; Hussong, Andrea; Kircher, Stefan; Kirchenbauer, Daniel; Timmer, Jens; Nagy, Ferenc; Schäfer, Eberhard; Fleck, Christian; Harmon, Frank [in: PLOS ONE]
Plants have evolved various sophisticated mechanisms to respond and adapt to changes of abiotic factors in their natural environment. Light is one of the most important abiotic environmental factors and it regulates plant growth and development throughout their entire life cycle. To monitor the intensity and spectral composition of the ambient light environment, plants have evolved multiple photoreceptors, including the red/far-red light-sensing phytochromes.
01A - Beitrag in wissenschaftlicher Zeitschrift
Bestimmung von Zersetzungskinetiken aus dynamischen Thermogrammen mittels eines Convolutional Neural Networks
(Hochschule für Life Sciences FHNW, 2023) Mensing, Dario; Zogg, Andreas; F. Hoffmann - La Roche
Um selbstreaktive Substanzen transportieren zu können muss eine sichere Transporttemperatur bestimmt werden. Als kritische Substanzen gelten Chemikalien mit einer Transporttemperatur unter 75 °C [1]. Die bestehenden Testmethoden (UN Test H.4) zur Ermittlung dieser Temperatur bringen lange Versuchszeiten mit sich und sind riskant da sie grosse Mengen an Testsubstanzen verwenden. Daher ist es wichtig, die Tests auf die kritischen Stoffe zu beschränken [2]. Ein entsprechender Workflow wurde von K. Wegmann während seiner Masterarbeit entwickelt. Die Schwierigkeit bei der Verarbeitung von DSC- und ARC-Daten liegt darin, dass die auftretenden Reaktionsmechanismen unbekannt sind. Entsprechend ist man bei Simulation auf ein einfache Reaktionsmodelle 0ter und nter Ordnung sowie modellfreie Kinetik beschränkt. [2]. In dieser Bachelorarbeit wurde daher eine Methode mittels eines Convolutional Neural Networks (CNN) entwickelt, um unterschiedliche Reaktionsmodelle von Zersetzungsreaktionen anhand von künstlich generierten DSC-Thermogrammen zu bestimmen. Bei einem CNN handelt es sich dabei um ein künstliches neurales Netzwerk, welches ursprünglich dazu entwickelt wurde komplexe Merkmale und Muster in Bildern zu erkennen [3].
11 - Studentische Arbeit
Carbon capture from flue gas of a chemical industrial park
(Hochschule für Life Sciences FHNW, 2023) Furrer, Thierry; Zogg, Andreas; Getec
In the chemical park Schweizerhalle, multiple incineration plants handle the hazardous waste generated by production processes. The ALV1 is the smaller one of the two waste solvent incinerators. Within the scope of this project, the feasibility to upgrade this plant with a carbon capture unit was assessed.
11 - Studentische Arbeit
Continuous in situ lactic acid extraction from sweet whey fermentation broth using a tubular membrane contactor
(2022) Demmelmayer, Paul; Pérez, Alan; Riedl, Wolfgang; Kienberger, Marlene
Background Whey, as the major dairy by-product, has become a severe problem for the dairy industry because its demand has decreased, while its production has increased in the past years. Since the high demand for dairy products tends to further increase, suitable routes for whey valorization are required. Whey contains valuables like lactate, but due its composition matrix, processing is challenging. The high sugar content in whey makes it a potential feedstock to produce lactic acid (LA) via microbial fermentation. Still, the known drawbacks of biotechnological LA production, such as growth inhibition by the product, the use of neutralizers, low productivity, and high overall costs of the process must be overcome. In situ LA recovering using a suitable separation technology is a potential strategy to overcome the mentioned drawbacks. In this work, the in situ LA extraction from a whey fermentation broth was evaluated. Sweet whey was used as feedstock for lactic acid production via microbial fermentation, while LA was recovered by reactive liquid-liquid extraction using a tubular membrane contactor. Method The feed material used in this work is a fermentation broth from sweet whey, which was filtered prior to extraction experiments using microfiltration. In a first series of experiments, two-phase extraction experiments were conducted in temperature controlled separation funnels, to investigate different solvent phase combinations for the lactic acid extraction. Special attention was paid to replace commonly applied fossil-based solvents with green alternatives, such as deep eutectic solvents (DES). After extraction, the extract was treated in a back-extraction step to recover LA from the solvent phase. Here, an anti-solvent, e.g. heptane or p-cymene, was used to transfer LA to the receiving phase, which was water. Afterwards the performance of reactive liquid-liquid extraction of LA using a PTFE membrane contactor was evaluated by using two potential solvent phases, namely tri-n-octyl amine (TOA) with n-decanol and a DES formed from thymol and menthol. From the experimental results the overall mass transfer coefficient was calculated based on a rigorous mass transfer model to predict the LA concentration in both phases. Finally, performance of the in situ LA recovery from sweet whey by using membrane contactor was investigated from both, experimentally and theoretically points of view. Results & Conclusion In the two-phase extraction experiments, the reactive extractants Aliquat336, TOA, trioctylphosphinoxid (TOPO), and the mixture Aliquat336/TOA were found to be most efficient with the diluents 1-decanol, limonene, and a deep eutectic solvent consisting of thymol and menthol. Especially, the DES and limonene are promising as they can be produced from renewable resources. With respect to back-extraction, heptane and p-cymene showed the highest efficiencies for transferring lactic acid into the receiving phase water. In the next step, the selected solvents were used to perform LA recovery in the tubular membrane contactor from a model aqueous solution of LA and a fermentation broth of sweet whey. The proposed technology for valorizing dairy waste through LA biotechnological production shows the feasibility to scaling it up.
06 - Präsentation
Corrigendum to “Powder cohesion and energy to break an avalanche. Can we address surface heterogeneity?” [Int. J. Pharm. 626 (2022) 122198]
(Elsevier, 2023) Brokešová, Jana; Niederquell, Andreas; Kuentz, Martin; Zámostný, Petr; Vraníková, Barbora; Šklubalová, Zdenka [in: International Journal of Pharmaceutics]
01A - Beitrag in wissenschaftlicher Zeitschrift
Design of a biocatalytic flow reactor based on hierarchically structured monolithic silica for producing galactooligosaccharides (GOSs)
(Schweizerische Chemische Gesellschaft, 2023) Dejoma, Riccardo; Buscemi, Andrea; Cutrona, Emilio; Shahgaldian, Patrick [in: CHIMIA]
Climate change mitigation requires the development of greener chemical processes. In this context, biocatalysis is a pivotal key enabling technology. The advantages of biocatalysis include lower energy consumption levels, reduced hazardous waste production and safer processes. The possibility to carry out biocatalytic reactions under flow conditions provides the additional advantage to retain the biocatalyst and to reduce costly downstream processes. Herein, we report a method to produce galactooligosaccharides (GOSs) from a largely available feedstock (i.e. lactose from dairy production) using a flow reactor based on hierarchically structured monolithic silica. This reactor allows for fast and efficient biotransformation reaction in flow conditions.
01A - Beitrag in wissenschaftlicher Zeitschrift
Development and validation of a liquid chromatography-triple quadrupole mass spectrometry method for the determination of isopeptide ε-(γ-glutamyl) lysine in human urine as biomarker for transglutaminase 2 cross-linked proteins
(Elsevier, 21.06.2023) Dejager, Lien; Jairaj, Mark; Jones, Kieran; Johnson, Timothy; Dudal, Sherri; Dudal, Yves; Shahgaldian, Patrick; Correro, Rita; Qu, Jun; An, Bo; Lucey, Richard; Szarka, Szabolcs; Wheller, Robert; Pruna, Alina; Kettell, Sarah; Pitt, Andrew; Cutler, Paul [in: Journal of Chromatography A]
01A - Beitrag in wissenschaftlicher Zeitschrift
Development of a novel measurement setup to study and predict electrostatic discharges in agitated glass‐lined vessels
(Wiley, 12.02.2024) Brönnimann, Benedikt; Egli‐Tedesco, Daniel; Schwenzfeuer, Klaus; Zogg, Andreas [in: Helvetica Chimica Acta]
Two glass lined reactors in a launch platform facility operated by Syngenta have been damaged during the crystallization of an organic compound due to electrostatic discharges. The goal of this work was to design and commission a novel setup to measure charges and currents generated by this slurry in a laboratory‐scale reactor. An improved and more sophisticated setup was then proposed for possible implementation in Syngenta's own laboratories. With this novel setup, the electrostatic charging of stirred suspensions involving nonconductive solvents could be accurately measured in the context of a case study that involved the suspension that led to liner damages in the production facilities of Syngenta.
01A - Beitrag in wissenschaftlicher Zeitschrift
Development of a tool for the automated visualization of batch recipes
(Hochschule für Life Sciences FHNW, 2024) Gentner, Jannick; Zogg, Andreas; Bachem AG
11 - Studentische Arbeit
Dynamics of early skin formation of tiling mortars investigated by microscopy and diffuse reflectance infrared Fourier transformed spectroscopy
(Elsevier, 03/2013) Bühler, Theodor; Zurbriggen, Roger; Pieles, Uwe; Huwiler, L.; Raso, Renzo [in: Cement and Concrete Composites]
A freshly applied mortar layer is exposed to the environment and forms a skin in the following minutes. Skinning changes significantly the surface properties and therefore limits application procedures. In the case of a tile adhesive, the skin that is formed before the tile is embedded can reduce resulting adhesion properties. Reflected light microscopy reveals that surface “lakes” disappear between 5 and 10 min after mortar application and DRIFTS (diffuse reflectance infrared Fourier transformed spectroscopy) indicates that films of organics and carbonates form simultaneously at the surface.
01A - Beitrag in wissenschaftlicher Zeitschrift
Ecotoxicity attenuation by acid-resistant nanofiltration in scandium recovery from TiO2 production waste
(Elsevier, 2023) Fekete-Kertész, Ildikó; Stirling, Tamás; Vaszita, Emese; Berkl, Zsófia; Farkas, Éva; Hedwig, Sebastian; Remmen, Kirsten; Lenz, Markus; Molnár, Mónika; Feigl, Viktória [in: Heliyon]
01A - Beitrag in wissenschaftlicher Zeitschrift
Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons
(Elsevier, 09/2023) Mungenast, Lena; Nieminen, Ronya; Gaiser, Carine; Faia-Torres, Ana Bela; Rühe, Jürgen; Suter-Dick, Laura [in: Biomaterials and Biosystems]
01A - Beitrag in wissenschaftlicher Zeitschrift
Energy-related chemical research at the universities of applied sciences
(Swiss Chemical Society, 2013) Riedl, Wolfgang; Fischer, Fabian; Marti, Roger; Brühwiler, Dominik [in: CHIMIA]
An overview of current activities in the field of energy-related chemical research at the Swiss Universities of Applied Sciences is presented.
01A - Beitrag in wissenschaftlicher Zeitschrift
Environmentally relevant UV-light weathering of polystyrene micro- and nanoplastics promotes hepatotoxicity in a human cell line
(Royal Society of Chemistry, 2023) Englert, Felix H.; Mueller, Fabrice A.; Dugershaw-Kurzer, Battuja; Kissling, Vera M.; Boentges, Sarah; Gupta, Govind S.; Fontana, Gabriele A.; Diedrich, Sabine; Suter-Dick, Laura; Sturla, Shana J.; Buerki-Thurnherr, Tina [in: Environmental Science: Nano]
Environmentally-relevant concentrations of UV-weathered polystyrene micro and nanoplastics induce hepatotoxicity and considerable changes in gene expression of liver disease-relevant pathways.
01A - Beitrag in wissenschaftlicher Zeitschrift
Enzymes for consumer products to achieve climate neutrality
(Oxford University Press, 2023) Molina-Espeja, Patricia; Sanz-Aparicio, Julia; Golyshin, Peter N.; Robles-Martín, Ana; Guallar, Víctor; Beltrametti, Fabrizio; Müller, Markus; Yakimov, Michail M.; Modregger, Jan; van Logchem, Moniec; Corvini, Philippe; Shahgaldian, Patrick; Degering, Christian; Wieland, Susanne; Timm, Anne; de Carvalho, Carla C. C. R.; Re, Ilaria; Daniotti, Sara; Thies, Stephan; Jaeger, Karl-Erich; Chow, Jennifer; Streit, Wolfgang R.; Lottenbach, Roland; Rösch, Rainer; Ansari, Nazanin; Ferrer, Manuel [in: Oxford Open Climate Change]
Today, the chemosphere’s and biosphere’s compositions of the planet are changing faster than experienced during the past thousand years. CO2 emissions from fossil fuel combustion are rising dramatically, including those from processing, manufacturing and consuming everyday products; this rate of greenhouse gas emission (36.2 gigatons accumulated in 2022) is raising global temperatures and destabilizing the climate, which is one of the most influential forces on our planet. As our world warms up, our climate will enter a period of constant turbulence, affecting more than 85% of our ecosystems, including the delicate web of life on these systems, and impacting socioeconomic networks. How do we deal with the green transition to minimize climate change and its impacts while we are facing these new realities? One of the solutions is to use renewable natural resources. Indeed, nature itself, through the working parts of its living systems, the enzymes, can significantly contribute to achieve climate neutrality and good ecological/biodiversity status. Annually they can help decreasing CO2 emissions by 1–2.5 billion-tons, carbon demand by about 200 million-tons, and chemical demand by about 90 million-tons. With current climate change goals, we review the consequences of climate change at multiple scales and how enzymes can counteract or mitigate them. We then focus on how they mobilize sustainable and greener innovations in consumer products that have a high contribution to global carbon emissions. Finally, key innovations and challenges to be solved at the enzyme and product levels are discussed.
01A - Beitrag in wissenschaftlicher Zeitschrift
Ex vivo drug testing in patient-derived papillary renal cancer cells reveals EGFR and the BCL2 family as therapeutic targets
(Elsevier, 2023) Angori, Silvia; Banaei-Esfahani, Amir; Mühlbauer, Katharina; Bolck, Hella A.; Kahraman, Abdullah; Karakulak, Tülay; Poyet, Cédric; Feodoroff, Michaela; Potdar, Swapnil; Kallioniemi, Olli; Pietiäinen, Vilja; Schraml, Peter; Moch, Holger [in: European Urology Focus]
01A - Beitrag in wissenschaftlicher Zeitschrift

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Auflistung Hochschule für Life Sciences FHNW nach Schlagwort "500 - Naturwissenschaften und Mathematik"

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