Listen
8 Ergebnisse
Ergebnisse nach Hochschule und Institut
Publikation Circularity and environmental sustainability of organic and printed electronics(Jenny Stanford Publishing, 2024) Le Blévennec, Kévin; Hengevoss, Dirk; Zimmermann, Yannick-Serge; Brun, Nadja; Hugi, Christoph; Lenz, Markus; Corvini, Philippe; Fent, Karl; Nisato, Giovanni; Lupo, Donald; Rudolf, SimoneIn this chapter, the possible role and impact of organic and printed electronics (OPE) in a transition toward a circular economy and more sustainable society will be discussed. The learning targets are twofold: first, understanding main environmental issues associated with the emerging field of OPE, and second, identifying, through a systemic perspective, the enabling potential of these technologies.04A - Beitrag SammelbandPublikation Mixtures of Aluminum and Indium Induce More than Additive Phenotypic and Toxicogenomic Responses in Daphnia magna(American Chemical Society, 01/2019) Brun, Nadja; Fent, KarlAquatic systems are contaminated by many metals but their effects as mixtures on organisms are not well understood. Here, we assessed effects of aluminum with fairly well-known modes of actions and indium, an understudied emerging contaminant from electronics, followed by studying equi-effective mixtures thereof. We report acute and adverse phenotypic effects in Daphnia magna adults and global transcriptomic effects employing RNA sequencing in neonates. The mixture induced more than additive activity in mortality and in physiological effects, including growth and reproduction. Similarly, transcriptomic effects were more than additive, as indicated by a markedly higher number of 463 differentially expressed transcripts in the mixture and by distinct classes of genes assigned to several biological functions, including metabolic processes, suggesting depleted energy reserves, which may be responsible for the observed impaired reproduction and growth. A gene set enrichment analysis (GSEA) of a priori known response pathways for aluminum confirmed activation of distinct molecular pathways by indium. Our study is highlighting more than additive effects at the transcriptional and physiological level and is providing a state-of-the art approach to mixture analysis, which is important for risk assessment of these metals and metal mixtures.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Cytotoxicity and molecular effects of biocidal disinfectants (quaternary ammonia, glutaraldehyde, poly(hexamethylene biguanide) hydrochloride PHMB) and their mixtures in vitro and in zebrafish eleuthero-embryos(Elsevier, 2017) Christen, Verena; Faltermann, Susanne; Fent, Karl; Brun, NadjaFrequently used biocidal disinfectants, including quaternary ammonium compounds (QAC), glutaraldehyde and poly(hexamethylene biguanide) hydrochloride (PHMB), occur in the aquatic environment but their potential effects in fish are poorly known, in particular when occurring as mixtures. To investigate their joint activity, we assessed the cytotoxicity of three QACs (BAC, barquat and benzalkonium chloride), glutaraldehyde andPHMB by the MTT assay individually, followed by assessing binary and ternary mixtures in zebrafish liver cells (ZFL) and human liver cells (Huh7). We also analysed molecular effects by quantitative PCR in vitro and in zebrafish eleuthero-embryos employing a targeted gene expression approach. QACs displayed strong cytotoxicity in both cell lines with EC50 values in the low μg/ml range, while glutaraldehyde and PHMB were less cytotoxic. Most of the binary and both ternary mixtures showed synergistic activity at all equi-effective concentrations. A mixture containing all five compounds mixed at their no observed effect concentrations showed strong cytotoxicity, suggesting a synergistic interaction. Additionally, we determined transcriptional alterations of target genes related to endoplasmatic reticulum (ER) stress, general stress, inflammatory action and apoptosis. Induction of ER stress genes occurred at non-cytotoxic concentrations of barquat, glutaraldehyde and BAC in ZFL cells. Barquat and BAC induced tumor necrosis factor alpha (tnf-α). Similar transcriptional alterations were found in vivo upon exposure of zebrafish eleuthero-embryos for 120 h. Glutaraldehyde led to induction of ER stress genes and tnf-α, while BAC additionally induced genes indicative of apoptosis, which was also the case with benzalkonium chloride at the highest concentration. We demonstrated strong cytotoxicity of QACs, and synergistic activity of binary, ternary and quintuple mixtures. Barquat and BAC let to induction of ER stress and inflammation in vitro, and BAC and glutaraldehyde at non-toxic concentrations in vivo, while benzalkonium chloride induced expression of tnf-α only.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Ecotoxicological assessment of solar cell leachates: Copper indium gallium selenide (CIGS) cells show higher activity than organic photovoltaic (OPV) cells(Elsevier, 01.02.2016) Brun, NadjaDespite the increasing use of photovoltaics their potential environmental risks are poorly understood. Here, we compared ecotoxicological effects of two thin-film photovoltaics: established copper indium gallium selenide (CIGS) and organic photovoltaic (OPV) cells. Leachates were produced by exposing photovoltaics to UV light, physical damage, and exposure to environmentally relevant model waters, representing mesotrophic lake water, acidic rain, and seawater. CIGS cell leachates contained 583 μg L(-1) molybdenum at lake water, whereas at acidic rain and seawater conditions, iron, copper, zinc, molybdenum, cadmium, silver, and tin were present up to 7219 μg L(-1). From OPV, copper (14 μg L(-1)), zinc (87 μg L(-1)) and silver (78 μg L(-1)) leached. Zebrafish embryos were exposed until 120 h post-fertilization to these extracts. CIGS leachates produced under acidic rain, as well as CIGS and OPV leachates produced under seawater conditions resulted in a marked hatching delay and increase in heart edema. Depending on model water and solar cell, transcriptional alterations occurred in genes involved in oxidative stress (cat), hormonal activity (vtg1, ar), metallothionein (mt2), ER stress (bip, chop), and apoptosis (casp9). The effects were dependent on the concentrations of cationic metals in leachates. Addition of ethylenediaminetetraacetic acid protected zebrafish embryos from morphological and molecular effects. Our study suggests that metals leaching from damaged CIGS cells, may pose a potential environmental risk.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Environmental aspects of printable and organic electronics (POE)(Pan Stanford Publishing, 04/2016) Hengevoss, Dirk; Zimmermann, Yannick; Brun, Nadja; Hugi, Christoph; Lenz, Markus; Corvini, Philippe; Fent, Karl; Nisato, Giovanni; Lupo, Donald; Ganz, Simone04A - Beitrag SammelbandPublikation Are degrading OPV materials still sustainable?(2015) Zimmermann, Yannick; Brun, Nadja; Hengevoss, Dirk; Corvini, Philippe; Fent, Karl; Hugi, Christoph; Lenz, Markus06 - PräsentationPublikation Transcriptional and phenotypic responses of Daphnia magna to aluminium, indium, and their binary mixtures(2015) Brun, Nadja; Mirbahai, Leda; Colbourne, John K .; Fent, Karl06 - PräsentationPublikation Ecotoxicological Assessment of Solar Cell Leachates(2015) Fent, Karl; Brun, Nadja; Wehrli, Bernhard06 - Präsentation