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Publikation Large‐scale eDNA monitoring of multiple aquatic pathogens as a tool to provide risk maps for wildlife diseases(Wiley, 11.05.2023) Sieber, Natalie; King, Alex; Krieg, Raphael; Zenker, Armin; Vorburger, Christoph; Hartikainen, HannaMultiple parasites and pathogens cause disease in aquatic wildlife and in aquaculture species, generating a need for monitoring and management. Conventional disease monitoring methods involve laborious, costly, and invasive capture and examination of host species, and require specialized expertise for every host and pathogen of interest. Environmental DNA could provide simultaneous occurrence data for multiple pathogens across different host taxa, valuable for using parasite diversity as, for example, a bioindicator of ecosystem disturbance. Here, we tested the potential for simultaneous detection of four wildlife pathogens in water samples from 280, mainly riverine, sites across Switzerland. We targeted the crayfish pathogen, the amphibian pathogen Batrachochytrium dendrobatidis, and the fish pathogens Saprolegnia parasitica and Tetracapsuloides bryosalmonae. The eDNA detection showed a widespread distribution of A. astaciS. parasitica T. bryosalmonae A. astaci and T. bryosalmonae were not detected in some alpine river catchments. B. dendrobatidis was detected only rarely, which was expected since the sampling did not target amphibian breeding sites. Co‐detection rates were higher in rivers than in lakes, likely reflecting the habitat preferences and distributions of the host species. We discuss the advantages and limitations of eDNA‐based pathogen monitoring and list a set of recommendations for managers. Our study illustrates how eDNA‐based techniques can monitor several pathogen species concurrently, thus facilitating more comprehensive disease monitoring schemes. Combined with metabarcoding approaches in the future, eDNA‐based sampling and detection can facilitate the incorporation of parasite and pathogen occurrence and diversity as an indicator for aquatic ecosystem health, and for revealing the hidden biodiversity and structure of parasite communities.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Measures to control invasive crayfish species in Switzerland: A success story?(Frontiers, 09.12.2020) Krieg, Raphael; King, Alex; Zenker, Armin01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Using eDNA to simultaneously detect the distribution of native and invasive crayfish within an entire country(Elsevier, 2022) Krieg, Raphael; Weston, Anna; Zenker, Armin; King, AlexThe introduction of invasive crayfish has led to a decline of many European native species of crayfish across their range. In this study, novel duplex assays for all crayfish occurring in Switzerland were developed. We aimed to identify the distribution of the seven species using a traditional trap surveillance method as well by collecting water samples to detect eDNA by species-specific quantitative real-time PCR. We reveal our overall experience in finding optimal field and laboratory techniques to discover the distribution and abundance of native and invasive species in order to enhance knowledge of early invasive species invasion and highlight important pockets of populations where native species remain, for implementation of conservation strategies. Using eDNA, important populations of native noble and white-clawed crayfish were revealed in multiple waters across various cantons. The successful identification of native and invasive crayfish species in Switzerland using eDNA can be applied to future nationwide projects. This method which has the ability to detect all species simultaneously across an entire country, will allow an improvement in freshwater crayfish conservation management.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Parasite DNA detection in water samples enhances crayfish plague monitoring in asymptomatic invasive populations(Springer, 01.11.2021) Sieber, Natalie; Hartikainen, Hanna; Krieg, Raphael; Zenker, Armin; Vorburger, ChristophInvasive species can facilitate the spread of pathogens by first providing asymptomatic host reservoirs, and then driving disease outbreaks in native populations through pathogen spillover. An example of this are invasive crayfish species in Europe (Faxonius limosus, Pacifastacus leniusculus, Procambarus clarkii), which carry the deadly plague agent (Aphanomyces astaci). Effective disease management requires comprehensive monitoring, however, pathogen detection in carrier populations with low pathogen prevalence and intensities is challenging. We simultaneously collected and analysed crayfish tissue samples of invasive crayfish populations and water samples to compare A. astaci detection in different sample types using quantitative PCR. Combined, the two sampling methods revealed A. astaci presence with DNA concentrations above limit of detection (LOD; the lowest concentration which can be detected with reasonable certainty) in 13 of 23 invasive crayfish populations. In four additional sites, A. astaci DNA concentrations below LOD were found in water. In four populations only were A. astaci concentrations above LOD detected in both sample types and in three populations in concentrations above LOD in tissue but below LOD in water. The likely reason for these discrepancies is the low A. astaci prevalence and concentration in resistant invasive crayfish, which limit detection reliability. Consistency may be improved by timing surveys with seasonal periods of high A. astaci abundance and by increasing water sampling effort. Considering the ease of collecting eDNA samples, compared to crayfish tissue sampling, eDNA methods would facilitate frequent and comprehensive surveys. However, remaining uncertainties in eDNA-based detection reveal the relevance of combining monitoring tools to improve detection of invasive pathogens and their management.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Barriers against invasive crayfish species in natural waters and fish passes. Practical experience(Elsevier, 01/2021) Krieg, Raphael; King, Alex; Zenker, ArminThe spread of non-indigenous crayfish species poses a threat to local populations of crayfish as well as to other fauna and flora across Europe and around the world. Several methods have been used in attempt to reduce their numbers and stop their further spread. Crayfish barriers are the best way to stop the non-anthropogenic spread of established invasive crayfish populations. Up to now there are very few published papers regarding crayfish barrier design and practical experience in construction. For the last seven years, we have optimised construction and functionality of crayfish barriers, tested various building materials and planned construction of many barriers across Switzerland. In this article, we highlight our experience, share the acquired knowledge and present the newest findings regarding considerations, which must be made when planning a barrier to stop the upstream movement of non-indigenous crayfish species. From our experience we conclude that crayfish barriers work in preventing the movement of invasive species if certain factors are taken into account. Barrier design and construction must be specific for each project, because the size of the watercourse, flow velocity, bank conditions, existing constructions and accessibility, all change the way a barrier should be set-up.01A - Beitrag in wissenschaftlicher Zeitschrift