Institut für Chemie und Bioanalytik

Dauerhafte URI für die Sammlunghttps://irf.fhnw.ch/handle/11654/24

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Autor:in: search.filters.author.Dalcanale, Federico

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  • Vorschaubild
    Publikation
    Apical medium flowiInfluences the morphology and physiology of human proximal tubular cells in a microphysiological system
    (MDPI, 30.09.2022) Specioso, Gabriele; Bovard, David; Zanetti, Filippo; Maranzano, Fabio; Merg, Céline; Sandoz, Antonin; Titz, Bjoern; Dalcanale, Federico; Hoeng, Julia; Renggli, Kasper; Suter-Dick, Laura
    There is a lack of physiologically relevant in vitro human kidney models for disease modelling and detecting drug-induced effects given the limited choice of cells and difficulty implementing quasi-physiological culture conditions. We investigated the influence of fluid shear stress on primary human renal proximal tubule epithelial cells (RPTECs) cultured in the micro-physiological Vitrofluid device. This system houses cells seeded on semipermeable membranes and can be connected to a regulable pump that enables controlled, unidirectional flow. After 7 days in culture, RPTECs maintained physiological characteristics such as barrier integrity, protein uptake ability, and expression of specific transporters (e.g., aquaporin-1). Exposure to constant apical side flow did not cause cytotoxicity, cell detachment, or intracellular reactive oxygen species accumulation. However, unidirectional flow profoundly affected cell morphology and led to primary cilia lengthening and alignment in the flow direction. The dynamic conditions also reduced cell proliferation, altered plasma membrane leakiness, increased cytokine secretion, and repressed histone deacetylase 6 and kidney injury molecule 1 expression. Cells under flow also remained susceptible to colistin-induced toxicity. Collectively, the results suggest that dynamic culture conditions in the Vitrofluid system promote a more differentiated phenotype in primary human RPTECs and represent an improved in vitro kidney model.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild
    Publikation
    3D printed microfluidic modules. Passive mixers and cells encapsulation in alginate
    (De Gruyter, 02.09.2022) Dalcanale, Federico; Caj, Michaela; Schuler, Felix; Ganeshanathan, Kireedan; Suter-Dick, Laura
    Passive mixers and droplet generation microfluidic chip modules were designed and manufactured using a commercial SLA 3D-printer. The mixing modules were designed specifically for 3D-printing and evaluated using FEM modeling. The co-flow droplet generator was used for cancer cells encapsulation and drug potency evaluation.
    01A - Beitrag in wissenschaftlicher Zeitschrift