Institut für Chemie und Bioanalytik
Dauerhafte URI für die Sammlunghttps://irf.fhnw.ch/handle/11654/24
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16 Ergebnisse
Ergebnisse nach Hochschule und Institut
Publikation Emerging Strategies for Ethanol Purification(Apple Academic Press, 2022) Pérez, Alan; Quintero, Javier; León, Juan A.; Hernández Almanza, Ayerim Y.; Balagurusamy, Nagamani; Ruiz Leza, Héctor; Aguilar, Cristóbal N.04A - Beitrag SammelbandPublikation Liquid extraction with immobilized liquids for product recovery from fermentation broths(Elsevier, 2022) Pérez, Alan; Gössi, Angelo; Riedl, Wolfgang; Schuur, Boelo; Fontalvo, Javier; Thatoi, Hrudayanath; Mohapatra, Sonali; Das, Swagat KumarNowadays, many fermentation chemicals are produced at an industrial scale. Numerous technological improvements have been developed and implemented to achieve high quality and quantity of fermentation products. However, several drawbacks in fermentation processes still limit their application at an industrial level. In situ product removal (ISPR) is a potential alternative to overcome the conventional drawbacks of the fermentative processes, increasing the fermentation's productivity and reducing the separation steps for recovery and purification. Currently, liquid extraction has emerged as a promising separation technology for ISPR, with immobilized liquids such as membrane-assisted extraction and microchannel liquid membrane, due to the high mass transfer rates, scalability, easy integration, and efficiency. This chapter will discuss these technologies regarding their integration into fermentative processes.04A - Beitrag SammelbandPublikation Environmentally relevant UV-light weathering of polystyrene micro- and nanoplastics promotes hepatotoxicity in a human cell line(Royal Society of Chemistry, 2023) Englert, Felix H.; Mueller, Fabrice A.; Dugershaw-Kurzer, Battuja; Kissling, Vera M.; Boentges, Sarah; Gupta, Govind S.; Fontana, Gabriele A.; Diedrich, Sabine; Suter-Dick, Laura; Sturla, Shana J.; Buerki-Thurnherr, TinaEnvironmentally-relevant concentrations of UV-weathered polystyrene micro and nanoplastics induce hepatotoxicity and considerable changes in gene expression of liver disease-relevant pathways.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation New scale-up technologies for multipurpose pharmaceutical production plants. Use case of a heterogeneous hydrogenation process(American Chemical Society, 06.07.2023) Furrer, Thierry; Levis, Michael; Berger, Bernhard; Kandziora, Maja; Zogg, Andreas01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Method to rapidly identify potential solvent systems for crystallization of cocrystals(American Chemical Society, 2023) Codan, Lorenzo; Daza Olivella, Laura; Sirota, Eric01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Development and validation of a liquid chromatography-triple quadrupole mass spectrometry method for the determination of isopeptide ε-(γ-glutamyl) lysine in human urine as biomarker for transglutaminase 2 cross-linked proteins(Elsevier, 21.06.2023) Dejager, Lien; Jairaj, Mark; Jones, Kieran; Johnson, Timothy; Dudal, Sherri; Dudal, Yves; Shahgaldian, Patrick; Correro, Rita; Qu, Jun; An, Bo; Lucey, Richard; Szarka, Szabolcs; Wheller, Robert; Pruna, Alina; Kettell, Sarah; Pitt, Andrew; Cutler, Paul01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Allosteric targeting resolves limitations of earlier LFA-1 directed modalities(Elsevier, 05/2023) Mancuso, Riccardo V.; Schneider, Gisbert; Hürzeler Müller, Marianne; Gut, Martin; Zurflüh, Jonas; Breitenstein, Werner; Bouitbir, Jamal; Reisen, Felix; Atz, Kenneth; Ehrhardt, Claus; Duthaler, Urs; Gygax, Daniel; Schmidt, Albrecht G.; Krähenbühl, Stephan; Weitz-Schmidt, Gabriele01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Determination of active ingredients in formulated plant protection products by UHPLC-UV/MS(Springer, 04.10.2022) Erdin, Yves; Schlotterbeck, Götz; Mink, ChristianThe market for plant protection products (PPPs) is one of the most regulated. With increasing regulations for registering PPPs, its requirements take more effort and more testing. Meanwhile, there is also an increasing market for illegal PPPs. While legally registered PPPs are thoroughly tested and documented, illegal PPPs are sold upfront without any testing (e.g., tox, aqua tox, eco tox). This bears additional risk for the users, the ecosystem and the public. There are many well established analytical methods and protocols to analyse soil, plant products as well as for food and beverage, but less for PPPs. Many methods need laborious sample preparation and extraction steps like QuEChERS and/or advanced mass spectrometry techniques. Here we present a method developed to identify active ingredients (AIs) which is easy to apply at relatively cheap costs and widely available instrumentation. It provides a straightforward set-up for sample preparation and analysis, without the need for elaborative sample preparation. The method validation showed sufficient linearity, repeatability and specificity to use this method for screening of samples taken from market control for counterfeit or cross contamination checks.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Electrochemical membrane-assisted pH-swing extraction and back-extraction of lactic acid(Elsevier, 2022) Gausmann, Marcel; Bertram, Franziska; Schuur, Boelo; Jupke, Andreas; Gössi, Angelo; Riedl, WolfgangReactive extraction of carboxylic acids such as lactic acid with tertiary amines is a state-of-the-art process but suffers strongly from reduced extraction efficiency in buffered environments like fermentation broths. In order to increase the efficiency of in-situ product removal, we here propose the combination of a membrane-assisted reactive extraction with an electrochemical pH shift. Prior to extraction in the membrane module, the fermentation broth containing the lactic acid at neutral pH is treated by anodic electrolysis to reduce the pH and thereby improve the extraction yield. Additionally, the cathodic reaction is used to increase the pH of the aqueous stream used for back-extraction of the loaded organic phase. Model solutions were used to develop a mathematical model, capable of calculating the required membrane area for in-situ extractions, considering the effect of the aqueous pH on the extraction performance. Additionally, using electrochemical pH shift, we were able to concentrate lactic acid from 1 wt% in the dilute broth to 7 wt% in the back extract.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Evaluation of dioxin induced transcriptomic responses in a 3D human liver microtissue model(Elsevier, 2022) Tian, Mingming; Gou, Xiao; Zhang, Xiaowei; Messner, Catherine; Suter-Dick, Laura; Yan, LuThree-dimensional human liver microtissue model provides a promising method for predicting the human hepatotoxicity of environmental chemicals. However, the dynamics of transcriptional responses of 3D human liver microtissue model to dioxins exposure remain unclear. Herein, time-series transcriptomic analysis was used to characterize modulation of gene expression over 14 days in 3D human liver microtissues exposed to 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD, 31 nM, 10 ng/ml). Changes in gene expression and modulation of biological pathways were evaluated at several time points. The results showed that microtissues stably expressed genes related to toxicological pathways (e.g. highly of genes involved in external stimuli and maintenance of cell homeostasis pathways) during the 14-day culture period. Furthermore, a weekly phenomenon pattern was observed for the number of the differentially expressed genes in microtissues exposed to TCDD at each time point. TCDD led to an induction of genes involved in cell cycle regulation at day three. Metabolic pathways were the main significantly induced pathways during the subsequent days, with the immune/inflammatory response enriched on the fifth day, and the cellular response to DNA damage was identified at the end of the exposure. Finally, relevant transcription patterns identified in microtissues were compared with published data on rodent and human cell-line studies to elucidate potential species-specific responses to TCDD over time. Cell development and cytochrome P450 pathway were mainly affected after a 3-day exposure, with the DNA damage response identified at the end of exposure in the human microtissue system but not in mouse/rat primary hepatocytes models. Overall, the 3D human liver microtissue model is a valuable tool to predict the toxic effects of environmental chemicals with a relatively long exposure.01A - Beitrag in wissenschaftlicher Zeitschrift