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Publikation Modulating the electronic structure of Mn promotes singlet oxygen generation from electrochemical oxidation of H₂O via O-O coupling(Elsevier, 12/2024) Chen, Hao; Hou, Zhiang; Yue, Jinzhu; Wang, Jinnan; Wang, Yi; Li, Aimin; Corvini, PhilippeSelectively catalytic conversion H₂O into singlet oxygen (¹O₂) without additional oxidants is considered as an economic-efficient method for organic pollutants degradation. However, H₂O are more consistent with the spin state of ¹O₂ than common oxygen (O₂), retarding the kinetics of spin transition-induced reaction between O₂ and ¹O₂. Herein, we report an unprecedented ¹O₂ mediated electrocatalytic oxidation process, which allows O–O coupling for ¹O₂ evolution from H₂O over CrMn@C anode. The electron occupancy (eg) of CrMn@C (0.89) is very close to the optimal eg (0.95) of manganese-based materials reported in the literature, which facilitates the activation of H₂O on surface. Mn(Mn0.193Cr1.808)O₄-Mn in CrMn@C electrode significantly promotes the activation of H₂O to produce *O, followed by coupling of *O at adjacent sites to produce *OO, which further spontaneously forms ¹O₂. And H₂¹⁸O isotope experiments provide direct evidence for the production of ¹O₂ directly from H₂O. Consequently, the production of ¹O₂ is enhanced with the yield of 785.6 μmol·L⁻¹. Such ¹O₂-dominated electrocatalytic oxidation system can achieve efficient removal of electron-rich pollutant (bisphenol A) and improve the biodegradability of pharmaceutical wastewater (from 0.17 to 0.39).01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Host cell protein networks as a novel co‐elution mechanism during protein. A chromatography(Wiley, 07.03.2024) Panikulam, Sherin; Hanke, Alexander; Kroener, Frieder; Karle, Anette; Anderka, Oliver; Villiger, Thomas; Lebesgue, NicolasHost cell proteins (HCPs) are process-related impurities of therapeutic proteins produced in for example, Chinese hamster ovary (CHO) cells. Protein A affinity chromatography is the initial capture step to purify monoclonal antibodies or Fc-based proteins and is most effective for HCP removal. Previously proposed mechanisms that contribute to co-purification of HCPs with the therapeutic protein are either HCP-drug association or leaching from chromatin heteroaggregates. In this study, we analyzed protein A eluates of 23 Fc-based proteins by LC-MS/MS to determine their HCP content. The analysis revealed a high degree of heterogeneity in the number of HCPs identified in the different protein A eluates. Among all identified HCPs, the majority co-eluted with less than three Fc-based proteins indicating a drug-specific co-purification for most HCPs. Only ten HCPs co-purified with over 50% of the 23 Fc-based proteins. A correlation analysis of HCPs identified across multiple protein A eluates revealed their co-elution as HCP groups. Functional annotation and protein interaction analysis confirmed that some HCP groups are associated with protein-protein interaction networks. Here, we propose an additional mechanism for HCP co-elution involving protein-protein interactions within functional networks. Our findings may help to guide cell line development and to refine downstream purification strategies.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Bi atom sharing Co-Bi₂O₂CO₃/BiOI S-scheme induced singlet oxygen-dominated photocatalytic oxidation system(Elsevier, 12/2024) Hou, Zhiang; Yue, Jinzhu; Chen, Hao; Wang, Jinnan; Li, Aimin; Corvini, PhilippeEndocrine disrupting chemicals (EDCs) interfere with the normal secretion, transport and metabolism of human hormones, thus affecting neurological, reproductive and immune functions. Photocatalysis is regarded as a facile organic degradation technique. The construction of heterojunctions can modulate the reactive oxygen species and enhance the photocatalytic performance of semiconductors. However, poor contact interfaces still severely limit carrier separation and transfer. Herein, we have doped Co to modulate the band structure of Bi₂O₂CO₃ while facilitating the in situ growth of BiOI on its surface via shared Bi atoms. This approach led to the development of a 2D/2D Co-Bi₂O₂CO₃/BiOI (Co-BOC/BiOI) S-scheme heterojunction characterized by atomically close contact interfaces. Furthermore, the photo-electrochemical characterization results indicate that the light adsorption capacity, carrier separation and transport efficiency of the optimized Co-BOC/BiOI-3 are greatly improved. This system demonstrates almost 100% removal rate for three typical EDCs within 60 min. The degradation kinetic constants show an improvement by an order of magnitude compared to single BiOI and Bi₂O₂CO₃. More importantly, O₂•﹣, which is produced from O₂ reduction on high negative conduction band, can be subsequently oxidized into 1O2 by photogenerated hole. Electron paramagnetic resonance and quenching experiments indicate that the organics degradation process is dominated by 1O2. This work offers new insights into the construction of high-quality S-scheme heterojunction interfaces for modulation of reactive oxygen species.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Maize (Zea mays L.) plants change their fate and accumulate non-extractable residues of sulfamethoxazole in the soil of arable land(American Chemical Society, 16.05.2024) Wu, Xuan; Sun, Feifei; Cao, Siqi; Wang, Qilin; Wang, Lianhong; Wang, Songfeng; He, Yan; Kolvenbach, Boris; Corvini, Philippe; Ji, RongThe fate of sulfonamide antibiotics in farmlands is crucial for food and ecological safety, yet it remains unclear. We used [phenyl-U-14C]-labeled sulfamethoxazole (14C-SMX) to quantitatively investigate the fate of SMX in a soil–maize system for 60 days, based on a six-pool fate model. Formation of nonextractable residues (NERs) was the predominant fate for SMX in unplanted soil, accompanied by minor mineralization. Notably, maize plants significantly increased SMX dissipation (kinetic constant kd = 0.30 day–1 vs 0.17 day–1), while substantially reducing the NER formation (92% vs 58% of initially applied SMX) and accumulating SMX (40%, mostly bound to roots). Significant NERs (maximal 29–42%) were formed via physicochemical entrapment (determined using silylation), which could partially be released and taken up by maize plants. The NERs consisted of a considerable amount of SMX formed via entrapment (1–8%) and alkali-hydrolyzable covalent bonds (2–12%, possibly amide linkage). Six and 10 transformation products were quantified in soil extracts and NERs, respectively, including products of hydroxyl substitution, deamination, and N-acylation, among which N-lactylated SMX was found for the first time. Our findings reveal the composition and instability of SMX-derived NERs in the soil–plant system and underscore the need to study the long-term impacts of reversible NERs.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Imaging in article therapy. Current practice and future trends(IOP Publishing, 06/2024) Paganelli, Chiara; Gianoli, Chiara; Knopf, Antje; Paganelli, Chiara; Gianoli, Chiara; Knopf, AntjeThe benefits of particle therapy (PT), stemming from its physical and radiobiological advantages, necessitate the use of dedicated imaging technologies and approaches to ensure precise treatment planning and delivery. This book endeavors to establish a fundamental understanding of imaging within the context of PT, alongside exploring current research and clinical perspectives on its role. The focus is directed towards examining near-room, in-room, and in-beam technologies, both those already in clinical use and those in the developmental stage, which play pivotal roles in treatment planning, delivery, and verification processes, enabling adjustments either offline or online. Concurrently, the text addresses methodological solutions derived from these imaging modalities to effectively address challenges such as range uncertainties, anatomical variations, and biological properties, thereby enhancing the accuracy of treatment modeling and adaptation.03 - SammelbandPublikation Conclusions and future perspectives of imaging in particle therapy(IOP Publishing, 06/2024) Paganelli, Chiara; Gianoli, Chiara; Knopf, Antje; Paganelli, Chiara; Gianoli, Chiara; Knopf, AntjeIn this chapter, we will consolidate the concluding remarks presented throughout the preceding chapters, with a specific emphasis on the technical and methodological advancements in image-guided particle therapy, as well as elucidating the future trajectory of this field.04A - Beitrag SammelbandPublikation Treatment verification in particle therapy(IOP Publishing, 01.06.2024) Gianoli, Chiara; De Simoni, M.; Knopf, Antje; Paganelli, Chiara; Gianoli, Chiara; Knopf, AntjeTo make the most of the physical and biological potentials of Particle Therapy (PT), it would be extremely desirable to verify dose deposition in vivo. In this chapter, we describe and compare in vivo range verification methods currently being proposed, developed or clinically implemented, including PET and prompt gamma imaging. The potential of other emerging techniques for indirect treatment verification will be also cited.04A - Beitrag SammelbandPublikation Integration of imaging in clinical protocols of particle therapy(IOP Publishing, 06/2024) Trnkova, P.; Bolsi, Alessandra; Knopf, Antje; Hoffmann, A.; Paganelli, Chiara; Gianoli, Chiara; Knopf, Antje04A - Beitrag SammelbandPublikation Electrochemical reconfiguration of NiFe layered double hydroxide on BiVO4 induced by black phosphorus quantum dots for promoting photoelectrochemical water splitting(Elsevier, 12/2024) Sun, Yunshuyu; Chen, Hao; Hou, Zhiang; Wang, Jinnan; Li, Aimin; Corvini, PhilippeIn this work, black phosphorus quantum dots (BPQDs) and NiFe layered double hydroxide (NiFe-LDHs) were loaded on BiVO4 photoanode for promoting photoelectrochemical (PEC) water splitting. As a narrow bandgap p-type semiconductor with high work function, BPQDs could not only form p-n junction with BiVO4 to enhance light harvest but also construct Schottky junction for charge carriers separation. More importantly, introduction of BPQDs facilitates the transformation of Ni(OH)2 into NiOOH so as to improve the water oxidation capacity at low bias voltage. Density functional theory (DFT) calculation demonstrates that introduction of BPQDs accelerates the α/γ phase transition dynamically and thermodynamically, thus BiVO4/BPQDs/NiFe-LDHs photoanode achieves photocurrent density of 5.28 mA cm−2 at 1.23 VRHE, AM 1.5 G and maintains good stability. In summary, this work provides new ideas for constructing efficient hole extraction layers on photoelectrode, well expanding the application of BPQDs in PEC water splitting.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Organ motion in particle therapy and the role of imaging(IOP Publishing, 06/2024) Paganelli, Chiara; Molinelli, Silvia; Knopf, Antje; Paganelli, Chiara; Gianoli, Chiara; Knopf, AntjeOrgan motion is one of the main challenges to account for in particle therapy to plan and deliver an accurate treatment. In this chapter, we will explain the concept of organ motion in terms of inter- and intra-fraction variations. The current inter- and intra-fraction motion compensation techniques demanding for imaging will be also reported.04A - Beitrag Sammelband