Ammann, Erik

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Ammann
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Erik
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Ammann, Erik

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20162
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Autor:in: Shahgaldian, Patrick × Hochschule: Hochschule für Life Sciences ×

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  • Vorschaubild nicht verfügbar
    Publikation
    Production of superparamagnetic nanobiocatalysts for green chemistry applications
    (Springer, 23.04.2016) Gasser, Christoph; Ammann, Erik; Schäffer, Andreas; Shahgaldian, Patrick; Corvini, Philippe [in: Applied Microbiology and Biotechnology]
    Immobilization of enzymes on solid supports is a convenient method for increasing enzymatic stability and enabling enzyme reuse. In the present work, a sorption-assisted surface conjugation method was developed and optimized to immobilize enzymes on the surface of superparamagnetic nanoparticles. An oxidative enzyme, i.e., laccase from Trametes versicolor was used as model enzyme. The immobilization method consists of the production of superparamagnetic nanoparticles by co-precipitation of FeCl2 and FeCl3. Subsequently, the particle surface is modified with an organosilane containing an amino group. Next, the enzymes are adsorbed on the particle surface before a cross-linking agent, i.e., glutaraldehyde is added which links the amino groups on the particle surface with the amino groups of the enzymes and leads to internal cross-linking of the enzymes as well. The method was optimized using response surface methodology regarding optimal enzyme and glutaraldehyde amounts, pH, and reaction times. Results allowed formulation of biocatalysts having high specific enzymatic activity and improved stability. The biocatalysts showed considerably higher stability compared with the dissolved enzymes over a pH range from 3 to 9 and in the presence of several chemical denaturants. To demonstrate the reusability of the immobilized enzymes, they were applied as catalysts for the production of a phenoxazinone dye. Virtually, 100 % of the precursor was transformed to the dye in each of the ten conducted reaction cycles while on average 84.5 % of the enzymatic activity present at the beginning of a reaction cycle was retained after each cycle highlighting the considerable potential of superparamagnetic biocatalysts for application in industrial processes.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Enzyme Shielding in an Enzyme-thin and Soft Organosilica Layer
    (Wiley, 2016) Correro, Maria Rita; Moridi, Negar; Schützinger, Hansjörg; Sykora, Sabine; Ammann, Erik; Peters, E. Henrik; Dudal, Yves; Corvini, Philippe; Shahgaldian, Patrick [in: Angewandte Chemie: International Edition]
    The fragile nature of most enzymes is a major hindrance to their use in industrial processes. Herein, we describe a synthetic chem. strategy to produce hybrid org.​/inorg. nanobiocatalysts; it exploits the self-​assembly of silane building blocks at the surface of enzymes to grow an organosilica layer, of controlled thickness, that fully shields the enzyme. Remarkably, the enzyme triggers a rearrangement of this organosilica layer into a significantly soft structure. We demonstrate that this change in stiffness correlates with the biocatalytic turnover rate, and that the organosilica layer shields the enzyme in a soft environment with a markedly enhanced resistance to denaturing stresses.
    01A - Beitrag in wissenschaftlicher Zeitschrift