Corvini, Philippe

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Corvini
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Philippe
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Corvini, Philippe

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2015 - 20165
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Enddatum: 2016 × Institut: Institut für Chemie und Bioanalytik ×

Suchergebnisse

Gerade angezeigt 1 - 5 von 5
  • Vorschaubild nicht verfügbar
    Publikation
    Supramolecular enzyme engineering in complex nanometer-thin biomimetic organosilica layers
    (Royal Society of Chemistry, 09/2016) Correro, Maria Rita; Takacs, Michael; Sykora, Sabine; Corvini, Philippe; Shahgaldian, Patrick [in: RSC Advances]
    The use of enzymes in industrial processes is often hampered by their limited stability under operational conditions. As enzymes' function and stability are directly correlated to their three-​dimensional structure, numerous methods aiming at the preservation of this structure have been developed. While stabilization can be achieved using solid scaffolds for encapsulating the enzyme, it often results in loss of enzymic activity owing to a lack of conformational mobility of the biocatalyst. With the idea of mimicking protein-​protein interactions to create a network of weak force interactions between the surface of an immobilized enzyme and a synthetic protective layer, we have developed a chem. strategy allowing the use of complex mixts. of building blocks mimicking the lateral chain of natural amino acids. After crosslinking a model enzyme at the surface of silica nanoparticles, incubation with eight different organosilane mixts. allowed growing protective organosilica layers of controlled thicknesses. The nanoparticles produced were characterized by SEM and their biocatalytic activity was measured under a series of operational stress conditions. Our results clearly demonstrated that increasing the complexity and biomimetic nature of the protection layer allowed for relevant improvement of the protection effect. Indeed, when compared with the basic formulation, selected complex formulations allowed for an improvement of up to 100​% when treated at 50 °C for 60 min or in the presence of a denaturing detergent (SDS)​.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Enzyme Shielding in an Enzyme-thin and Soft Organosilica Layer
    (Wiley, 2016) Correro, Maria Rita; Moridi, Negar; Schützinger, Hansjörg; Sykora, Sabine; Ammann, Erik; Peters, E. Henrik; Dudal, Yves; Corvini, Philippe; Shahgaldian, Patrick [in: Angewandte Chemie: International Edition]
    The fragile nature of most enzymes is a major hindrance to their use in industrial processes. Herein, we describe a synthetic chem. strategy to produce hybrid org.​/inorg. nanobiocatalysts; it exploits the self-​assembly of silane building blocks at the surface of enzymes to grow an organosilica layer, of controlled thickness, that fully shields the enzyme. Remarkably, the enzyme triggers a rearrangement of this organosilica layer into a significantly soft structure. We demonstrate that this change in stiffness correlates with the biocatalytic turnover rate, and that the organosilica layer shields the enzyme in a soft environment with a markedly enhanced resistance to denaturing stresses.
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    A cyclodextrin-based polymer (CDP) for sensing diclofenac in water
    (Elsevier, 15.12.2015) Xiao, Pu; Weibel, Nicolas; Dudal, Yves; Corvini, Philippe; Shahgaldian, Patrick [in: Journal of Hazardous Materials]
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Reversible Supramolecular Surface Attachment of Enzyme–Polymer Conjugates for the Design of Biocatalytic Filtration Membranes
    (Wiley, 01.12.2015) Moridi, Negar; Corvini, Philippe; Shahgaldian, Patrick [in: Angewandte Chemie: International Edition]
    01A - Beitrag in wissenschaftlicher Zeitschrift
  • Vorschaubild nicht verfügbar
    Publikation
    Virus-like particles as virus substitutes to design artificial virus-recognition nanomaterials
    (Royal Society of Chemistry, 05.01.2015) Sykora, Sabine; Belliot, Gaël; Pothier, Pierre; Cumbo, Alessandro; Arnal, Charlotte; Dudal, Yves; Corvini, Philippe; Shahgaldian, Patrick [in: Chemical Communications]
    Functional recognition imprints of virus-like particles, at the surface of silica particles, were generated following a strategy based on protein-templated polycondensation of organosilanes.
    01A - Beitrag in wissenschaftlicher Zeitschrift