Analysing and quantifying chronic stress‐associated endogenous steroids in hair samples

Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Drug Testing and Analysis
Special issue
DOI of the original publication
Link
Series
Series number
Volume
Issue / Number
Pages / Duration
Patent number
Publisher / Publishing institution
Wiley
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
In previous studies, various steroids have been associated with stress and have therefore been quantified to investigate stress‐related questions. Since the main stress‐related steroid cortisol follows a circadian rhythm, often hair is analysed to quantify this steroid. Further, hair analysis gives the unique possibility of long‐time monitoring by analysing a certain segment of hair, since hair grows on average 1 cm per month. Hair is a difficult matrix due to the complex sample preparation with many steps including washing and grinding, followed by various extraction steps. Additionally, steroids are endogenous and are therefore present in the hair matrix. Hence, no analyte free matrix is available, which is needed for the quantification via external calibrators. To overcome this problem, the so‐called surrogate methods can be used, for which a 13 C 3 labelled or deuterated reference compound of the steroid of interest is used for quantification. In the present study, a surrogate method was developed and fully validated for the quantitative analysis of seven steroids in human hair. Validation experiments showed that the method is further suitable for semi‐quantitative analysis of estradiol. However, it is not suitable for the analysis of androsterone and DHEAS. The method was successfully used to analyse steroids in a comprehensive study of 360 adolescent hair samples, enabling research into stress markers.
Keywords
Stress, Haircortisol
Subject (DDC)
Project
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
1942-7603
1942-7611
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Hybrid
License
'https://creativecommons.org/licenses/by-nc/4.0/'
Citation
GRAFINGER, Katharina, Wassilis KASSIS, Céline Anne FAVRE, Dilan AKSOY und Stefan GAUGLER, 2024. Analysing and quantifying chronic stress‐associated endogenous steroids in hair samples. Drug Testing and Analysis. 14 März 2024. DOI 10.1002/dta.3678. Verfügbar unter: https://doi.org/10.26041/fhnw-8593