Analysing and quantifying chronic stress‐associated endogenous steroids in hair samples
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Autor:in (Körperschaft)
Publikationsdatum
14.03.2024
Typ der Arbeit
Studiengang
Typ
01A - Beitrag in wissenschaftlicher Zeitschrift
Herausgeber:innen
Herausgeber:in (Körperschaft)
Betreuer:in
Übergeordnetes Werk
Drug Testing and Analysis
Themenheft
DOI der Originalpublikation
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Reihe / Serie
Reihennummer
Jahrgang / Band
Ausgabe / Nummer
Seiten / Dauer
Patentnummer
Verlag / Herausgebende Institution
Wiley
Verlagsort / Veranstaltungsort
Auflage
Version
Programmiersprache
Abtretungsempfänger:in
Praxispartner:in/Auftraggeber:in
Zusammenfassung
In previous studies, various steroids have been associated with stress and have therefore been quantified to investigate stress‐related questions. Since the main stress‐related steroid cortisol follows a circadian rhythm, often hair is analysed to quantify this steroid. Further, hair analysis gives the unique possibility of long‐time monitoring by analysing a certain segment of hair, since hair grows on average 1 cm per month. Hair is a difficult matrix due to the complex sample preparation with many steps including washing and grinding, followed by various extraction steps. Additionally, steroids are endogenous and are therefore present in the hair matrix. Hence, no analyte free matrix is available, which is needed for the quantification via external calibrators. To overcome this problem, the so‐called surrogate methods can be used, for which a 13 C 3 labelled or deuterated reference compound of the steroid of interest is used for quantification. In the present study, a surrogate method was developed and fully validated for the quantitative analysis of seven steroids in human hair. Validation experiments showed that the method is further suitable for semi‐quantitative analysis of estradiol. However, it is not suitable for the analysis of androsterone and DHEAS. The method was successfully used to analyse steroids in a comprehensive study of 360 adolescent hair samples, enabling research into stress markers.
Schlagwörter
Stress, Haircortisol
Fachgebiet (DDC)
Veranstaltung
Startdatum der Ausstellung
Enddatum der Ausstellung
Startdatum der Konferenz
Enddatum der Konferenz
Datum der letzten Prüfung
ISBN
ISSN
1942-7603
1942-7611
1942-7611
Sprache
Englisch
Während FHNW Zugehörigkeit erstellt
Ja
Publikationsstatus
Veröffentlicht
Begutachtung
Peer-Review der ganzen Publikation
Open Access-Status
Hybrid
Lizenz
Zitation
GRAFINGER, Katharina, Wassilis KASSIS, Céline Anne FAVRE, Dilan AKSOY und Stefan GAUGLER, 2024. Analysing and quantifying chronic stress‐associated endogenous steroids in hair samples. Drug Testing and Analysis. 14 März 2024. DOI 10.1002/dta.3678. Verfügbar unter: https://doi.org/10.26041/fhnw-8593