Quantifying origins of cell-to-cell variations in gene expression
No Thumbnail Available
Authors
Kollmann, Markus
Author (Corporation)
Publication date
15.11.2008
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Biophysical Journal
Special issue
DOI of the original publication
Link
Series
Series number
Volume
95
Issue / Number
10
Pages / Duration
4523-4528
Patent number
Publisher / Publishing institution
Cell Press
Place of publication / Event location
Edition
Version
Programming language
Assignee
Practice partner / Client
Abstract
A general dynamic description of protein synthesis was employed to quantify different sources of gene expression noise in cellular systems. To test our approach, we use time-resolved expression data of individual human cells and, from this information, predict the stationary cell-to-cell variation in protein levels in a clonal population. For three of the four human genes investigated, the cellular variations in expression level are not due to fluctuations in promoter activity or transcript copy number, but are almost exclusively a consequence of long-term variations of gene regulatory factors or the global cellular state. Moreover, we show that a dynamic description is much more reliable to discriminate extrinsic and intrinsic sources of noise than it is on grounds of cell-cycle averaged descriptions. The excellent agreement between the theoretical predictions and the experimentally measured noise strengths shows that a quantitative description of gene expression noise is indeed possible on the basis of idealized stochastic processes.
Keywords
Subject (DDC)
600 - Technik, Medizin, angewandte Wissenschaften
Event
Exhibition start date
Exhibition end date
Conference start date
Conference end date
Date of the last check
ISBN
ISSN
0006-3495
1542-0086
1542-0086
Language
English
Created during FHNW affiliation
No
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Closed
License
Citation
RAUSENBERGER, Julia und Markus KOLLMANN, 2008. Quantifying origins of cell-to-cell variations in gene expression. Biophysical Journal. 15 November 2008. Bd. 95, Nr. 10, S. 4523–4528. DOI 10.1529/biophysj.107.127035. Verfügbar unter: https://irf.fhnw.ch/handle/11654/45743