Towards 3D bioprinted spinal cord organoids
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Authors
Han, Yilin
King, Marianne
Tikhomirov, Evgenii
Barasa, Povilas
Dos Santos Souza, Cleide
Lindh, Jonas
Baltriukiene, Daiva
Ferraiuolo, Laura
Azzouz, Mimoun
Author (Corporation)
Publication date
21.05.2022
Typ of student thesis
Course of study
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
International Journal of Molecular Sciences
Special issue
DOI of the original publication
Link
Series
Series number
Volume
23
Issue / Number
10
Pages / Duration
1-13
Patent number
Publisher / Publishing institution
MDPI
Place of publication / Event location
Edition
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Practice partner / Client
Abstract
Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation.
Keywords
Cell survival, Cell differentiation, Hydrogel, Bioprinting
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ISBN
ISSN
1422-0067
1661-6596
1661-6596
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
Gold
License
Citation
Han, Y., King, M., Tikhomirov, E., Barasa, P., Dos Santos Souza, C., Lindh, J., Baltriukiene, D., Ferraiuolo, L., Azzouz, M., Gullo, M., & Kozlova, E. N. (2022). Towards 3D bioprinted spinal cord organoids. International Journal of Molecular Sciences, 23(10), 1–13. https://doi.org/10.3390/ijms23105788