In vitro modulation of inflammatory target gene expression by a polyphenol-enriched fraction of rose oil distillation waste water
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Authors
Author (Corporation)
Publication date
2016
Typ of student thesis
Course of study
Collections
Type
01A - Journal article
Editors
Editor (Corporation)
Supervisor
Parent work
Fitoterapia
Special issue
DOI of the original publication
Link
Series
Series number
Volume
114
Issue / Number
Pages / Duration
56-62
Patent number
Publisher / Publishing institution
Elsevier
Place of publication / Event location
Edition
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Abstract
Classical production of rose oil is based on water steam distillation from the flowers of Rosa damascena. During this process, large quantities of waste water accrue which are discharged to the environment, causing severe pollution of both, groundwater and surface water due to a high content of polyphenols. We recently developed a strategy to purify the waste water into a polyphenol-depleted and a polyphenol-enriched fraction RF20-(SP-207). RF20-(SP-207) and sub-fraction F(IV) significantly inhibited cell proliferation and migration of HaCaT cells. Since there is a close interplay between these actions and inflammatory processes, here we focused on the fractions' influence on pro-inflammatory biomarkers. HaCaT keratinocytes were treated with RF20-(SP-207), F(IV) (both at 50 μg/mL) and ellagic acid (10 μM) for 24 h under TNF-α (20 ng/mL) stimulated and non-stimulated conditions. Gene expression of IL-1β, IL-6, IL-8, RANTES and MCP-1 was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and cellular protein secretion of IL-8, RANTES and MCP-1 was determined by ELISA based assays. RF20-(SP-207) and F(IV) significantly decreased the expression and cellular protein secretion of IL-1β, IL-6, IL-8, RANTES and MCP-1. The diminishing effects on inflammatory target gene expression were slightly less pronounced under TNF-α stimulated conditions. In conclusion, the recovered polyphenol fraction RF20-(SP-207) from rose oil distillation waste water markedly modified inflammatory target gene expression in vitro, and, therefore, could be further developed as alternative treatment of acute and chronic inflammation.
Keywords
Anti-inflammatory, ELISA, RT-PCR, Rosa damascena, Waste water
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ISBN
ISSN
1873-6971
0367-326X
0367-326X
Language
English
Created during FHNW affiliation
Yes
Strategic action fields FHNW
Publication status
Published
Review
Peer review of the complete publication
Open access category
License
Citation
Weston, A., Rausenberger, J., Butterweck, V., & Wedler, J. (2016). In vitro modulation of inflammatory target gene expression by a polyphenol-enriched fraction of rose oil distillation waste water. Fitoterapia, 114, 56–62. https://doi.org/10.1016/j.fitote.2016.08.019