Assessing the biodegradation of btex and stress response in a bio-permeable reactive barrier using compound-specific isotope analysis

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Authors
Chen, Tianyu
Wu, Yan
Wang, Jinnan
Author (Corporation)
Publication date
20.07.2022
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01A - Journal article
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International Journal of Environmental Research and Public Health
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Volume
19
Issue / Number
14
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MDPI
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Abstract
By using compound-specific isotope analysis (CSIA) in combination with high-throughput sequencing analysis (HTS), we successfully evaluated the benzene and toluene biodegradation in a bio-permeable reactive barrier (bio-PRB) and the stress response of the microbial community. Under stress conditions, a greater decline in the biodegradation rate of BTEX was observed compared with the apparent removal rate. Both an increase in the influent concentration and the addition of trichloroethylene (TCE) inhibited benzene biodegradation, while toluene biodegradation was inhibited by TCE. Regarding the stress response, the relative abundance of the dominant bacterial community responsible for the biodegradation of BTEX increased with the influent concentration. However, the dominant bacterial community did not change, and its relative abundance was restored after the influent concentration decreased. On the contrary, the addition of TCE significantly changed the bacterial community, with Aminicenantes becoming the dominant phyla for co-metabolizing TCE and BTEX. Thus, TCE had a more significant influence on the bio-PRB than an increasing influent concentration, although these two stress conditions showed a similar degree of influence on the apparent removal rate of benzene and toluene. The present work not only provides a new method for accurately evaluating the biodegradation performance and microbial community in a bio-PRB, but also expands the application of compound-specific isotope analysis in the biological treatment of wastewater.
Keywords
bio-permeable reactive barrier, biodegradation, BTEX, compound-specific isotope analysis, high-throughput sequencing analysis
Subject (DDC)
500 - Naturwissenschaften
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1661-7827
1660-4601
Language
English
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Yes
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Publication status
Published
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Peer review of the complete publication
Open access category
Gold
License
'https://creativecommons.org/licenses/by/4.0/'
Citation
CHEN, Tianyu, Yan WU, Jinnan WANG und Philippe CORVINI, 2022. Assessing the biodegradation of btex and stress response in a bio-permeable reactive barrier using compound-specific isotope analysis. International Journal of Environmental Research and Public Health. 20 Juli 2022. Bd. 19, Nr. 14. DOI 10.3390/ijerph19148800. Verfügbar unter: https://doi.org/10.26041/fhnw-4320