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Publikation Analyzing microbial communities and their biodegradation of multiple pharmaceuticals in membrane bioreactors(Springer, 12.07.2023) Suleiman, Marcel; Demaria, Francesca; Zimmardi, Cristina; Kolvenbach, Boris; Corvini, PhilippeAbstract Pharmaceuticals are of concern to our planet and health as they can accumulate in the environment. The impact of these biologically active compounds on ecosystems is hard to predict, and information on their biodegradation is necessary to establish sound risk assessment. Microbial communities are promising candidates for the biodegradation of pharmaceuticals such as ibuprofen, but little is known yet about their degradation capacity of multiple micropollutants at higher concentrations (100 mg/L). In this work, microbial communities were cultivated in lab-scale membrane bioreactors (MBRs) exposed to increasing concentrations of a mixture of six micropollutants (ibuprofen, diclofenac, enalapril, caffeine, atenolol, paracetamol). Key players of biodegradation were identified using a combinatorial approach of 16S rRNA sequencing and analytics. Microbial community structure changed with increasing pharmaceutical intake (from 1 to 100 mg/L) and reached a steady-state during incubation for 7 weeks on 100 mg/L. HPLC analysis revealed a fluctuating but significant degradation (30–100%) of five pollutants (caffeine, paracetamol, ibuprofen, atenolol, enalapril) by an established and stable microbial community mainly composed of Achromobacter, Cupriavidus, Pseudomonas and Leucobacter. By using the microbial community from MBR1 as inoculum for further batch culture experiments on single micropollutants (400 mg/L substrate, respectively), different active microbial consortia were obtained for each single micropollutant. Microbial genera potentially responsible for degradation of the respective micropollutant were identified, i.e. Pseudomonas sp. and Sphingobacterium sp. for ibuprofen, caffeine and paracetamol, Sphingomonas sp. for atenolol and Klebsiella sp. for enalapril. Our study demonstrates the feasibility of cultivating stable microbial communities capable of degrading simultaneously a mixture of highly concentrated pharmaceuticals in lab-scale MBRs and the identification of microbial genera potentially responsible for the degradation of specific pollutants. Key points • Multiple pharmaceuticals were removed by stable microbial communities. • Microbial key players of five main pharmaceuticals were identified.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Insights into the applications of 3D bioprinting for bioremediation technologies(Elsevier, 2021) Ke, Zhuang; Obamwonyi, Osagie; Kolvenbach, Boris; Ji, Rong; Liu, Shuangjiang; Jiang, Jiandong; Corvini, PhilippeA plethora of organic pollutants such as pesticides, polycyclic and halogenated aromatic hydrocarbons, and emerging pollutants, such as flame retardants, is continuously being released into the environment. This poses a huge threat to the society in terms of environmental pollution, agricultural product quality, and general safety. Therefore, effective removal of organic pollutants from the environment has become an important challenge to be addressed. As a consequence of the recent and rapid developments in additive manufacturing, 3D bioprinting technology is playing an important role in the pharmaceutical industry. At the same time, an increasing number of microorganisms suitable for the production of biomaterials with complex structures and functions using 3D bioprinting technology, have been identified. This article briefly discusses the principles, advantages, and disadvantages of different 3D bioprinting technologies for pollutant removal. Furthermore, the feasibility and challenges of developing bioremediation technologies based on 3D bioprinting have also been discussed01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Biodeterioration affecting efficiency and lifetime of plastic-based photovoltaics(Elsevier, 16.09.2020) Schmidt, Felix; Lenz, Markus; Schaeffer, Andreas; Zimmermann, Yannick; Alves dos Reis Benatto, Gisele; Kolvenbach, Boris; Krebs, FrederikThe low environmental impact of electricity generation using solar cells crucially depends on high energy-conversion efficiencies, long lifetimes and a minimal energy and material demand during production. Emerging thin-film photovoltaics such as perovskites on plastic substrates could hold promise to fulfil all these requirements. Under real-world operating conditions photovoltaic operation is challenged by biological stressors, which have not been incorporated for evaluation in any test. Such stressors cause biodeterioration, which impairs diverse, apparently inert materials such as rock, glass and steel and therefore could significantly affect the function and stability of plastic-based solar cells. Given that different photovoltaic technologies commonly use similar materials, the biodeterioration mechanisms reviewed here may possibly affect the efficiency and lifetimes of several technologies, if they occur sufficiently fast (during the expected lifetime of photovoltaics). Once the physical integrity of uppermost module layers is challenged by biofilm growth microbially mediated dissolution and precipitation reactions of photovoltaic functional materials are very likely to occur. The biodeterioration of substrates and seals also represents emission points for the release of potentially harmful photovoltaic constituents to the environment01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Biodegradation of sulfamethoxazole by a bacterial consortium of Achromobacter denitrificans PR1 and Leucobacter sp. GP(Springer, 12/2018) Reis, Ana C.; Cvancarova Småstuen, M.; Liu, Ying; Lenz, Markus; Hettich, Timm; Kolvenbach, Boris; Corvini, Philippe; Nunes, Olga C.In the last decade, biological degradation and mineralization of antibiotics have been increasingly reported feats of environmental bacteria. The most extensively described example is that of sulfonamides that can be degraded by several members of Actinobacteria and Proteobacteria. Previously, we reported sulfamethoxazole (SMX) degradation and partial mineralization by Achromobacter denitrificans strain PR1, isolated from activated sludge. However, further studies revealed an apparent instability of this metabolic trait in this strain. Here, we investigated this instability and describe the finding of a low-abundance and slow-growing actinobacterium, thriving only in co-culture with strain PR1. This organism, named GP, shared highest 16S rRNA gene sequence similarity (94.6–96.9%) with the type strains of validly described species of the genus Leucobacter. This microbial consortium was found to harbor a homolog to the sulfonamide monooxygenase gene (sadA) also found in other sulfonamide-degrading bacteria. This gene is overexpressed in the presence of the antibiotic, and evidence suggests that it codes for a group D flavin monooxygenase responsible for the ipso-hydroxylation of SMX. Additional side reactions were also detected comprising an NIH shift and a Baeyer–Villiger rearrangement, which indicate an inefficient biological transformation of these antibiotics in the environment. This work contributes to further our knowledge in the degradation of this ubiquitous micropollutant by environmental bacteria.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Ipso-substitution--the hidden gate to xenobiotic degradation pathways(Elsevier, 2015) Ricken, Benjamin; Kolvenbach, Boris; Corvini, Philippe01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Degradation of sulfonamide antibiotics by Microbacterium sp. strain BR1 - elucidating the downstream pathway(Elsevier, 2015) Ricken, Benjamin; Fellmann, Oliver; Kohler, Hans-Peter E.; Schäffer, Andreas; Corvini, Philippe; Kolvenbach, Boris01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Biomineralisierung von Selen: Von Abwasserbehandlung zu Ressourcen-Wiedergewinnung(2015) Lenz, Markus; Kolvenbach, Boris; Corvini, Philippe06 - PräsentationPublikation Enhanced Transformation of Tetrabromobisphenol A by Nitrifiers in Nitrifying Activated Sludge(American Chemical Society, 2015) Li, Fangjie; Jiang, Bingqi; Nastold, Peter; Kolvenbach, Boris; Chen, Jianqiu; Wang, Lianhong; Guo, Hongyan; Corvini, Philippe; Ji, Rong01A - Beitrag in wissenschaftlicher ZeitschriftPublikation New insights into biological sulfonamide degradation(2015) Kolvenbach, Boris; Ricken, Benjamin; Fellmann, Oliver; Kohler, Hans-Peter E.; Schäffer, Andreas; Corvini, Philippe06 - PräsentationPublikation Insights into the sulfonamide degrading protein complex(2015) Ricken, Benjamin; Bucher, Andreas; Mariossi, Andrea; Fellmann, Oliver; Adaixo, Ricardo; Schäffer, Andreas; Corvini, Philippe; Kolvenbach, Boris; Kohler, Hans-Peter E.; Leu, Cedric06 - Präsentation