Institut für Ecopreneurship

Dauerhafte URI für die Sammlunghttps://irf.fhnw.ch/handle/11654/26

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  • Publikation
    An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin
    (Royal Society of Chemistry, 04/2018) Zhao, Jingming; Bachmann, Daniel G.; Lenz, Markus; Gillingham, Dennis G.; Ward, Thomas R.
    We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers).
    01A - Beitrag in wissenschaftlicher Zeitschrift