DSpace

Background Whey, as the major dairy by-product, has become a severe problem for the dairy industry because its demand has decreased, while its production has increased in the past years. Since the high demand for dairy products tends to further increase, suitable routes for whey valorization are required. Whey contains valuables like lactate, but due its composition matrix, processing is challenging. The high sugar content in whey makes it a potential feedstock to produce lactic acid (LA) via microbial fermentation. Still, the known drawbacks of biotechnological LA production, such as growth inhibition by the product, the use of neutralizers, low productivity, and high overall costs of the process must be overcome. In situ LA recovering using a suitable separation technology is a potential strategy to overcome the mentioned drawbacks. In this work, the in situ LA extraction from a whey fermentation broth was evaluated. Sweet whey was used as feedstock for lactic acid production via microbial fermentation, while LA was recovered by reactive liquid-liquid extraction using a tubular membrane contactor. Method The feed material used in this work is a fermentation broth from sweet whey, which was filtered prior to extraction experiments using microfiltration. In a first series of experiments, two-phase extraction experiments were conducted in temperature controlled separation funnels, to investigate different solvent phase combinations for the lactic acid extraction. Special attention was paid to replace commonly applied fossil-based solvents with green alternatives, such as deep eutectic solvents (DES). After extraction, the extract was treated in a back-extraction step to recover LA from the solvent phase. Here, an anti-solvent, e.g. heptane or p-cymene, was used to transfer LA to the receiving phase, which was water. Afterwards the performance of reactive liquid-liquid extraction of LA using a PTFE membrane contactor was evaluated by using two potential solvent phases, namely tri-n-octyl amine (TOA) with n-decanol and a DES formed from thymol and menthol. From the experimental results the overall mass transfer coefficient was calculated based on a rigorous mass transfer model to predict the LA concentration in both phases. Finally, performance of the in situ LA recovery from sweet whey by using membrane contactor was investigated from both, experimentally and theoretically points of view. Results & Conclusion In the two-phase extraction experiments, the reactive extractants Aliquat336, TOA, trioctylphosphinoxid (TOPO), and the mixture Aliquat336/TOA were found to be most efficient with the diluents 1-decanol, limonene, and a deep eutectic solvent consisting of thymol and menthol. Especially, the DES and limonene are promising as they can be produced from renewable resources. With respect to back-extraction, heptane and p-cymene showed the highest efficiencies for transferring lactic acid into the receiving phase water. In the next step, the selected solvents were used to perform LA recovery in the tubular membrane contactor from a model aqueous solution of LA and a fermentation broth of sweet whey. The proposed technology for valorizing dairy waste through LA biotechnological production shows the feasibility to scaling it up.

The application of a liquid membrane in Taylor flow (LMTF) is a promising method that can be integrated with other separation or reactive processes in view of process intensification. In this work, a model for a hybrid LMTF – fermentation system was developed for lactic acid production using batch fermentation and LMTF experimental data. The hybrid model is compared to experimental data of the hybrid system. Through a sensitivity analysis of the main variables of the LMTF an optimum value of the overall volumetric mass transfer coefficient (0.0122 1/s) was achieved for lactic acid removal. This was further used for modeling the hybrid system. The fermentation time of the hybrid system is reduced by 7 h, the productivity and biomass concentration is increased by 2.578 g/(L·h) and 2.7016 g/L, respectively, as compared with a batch fermentation. In addition, the effect of the number of channels of the LMTF is modeled and its impact on productivity, fermentation time, and final biomass concentration is analyzed. It was concluded that lactic acid removal through the LMTF from the fermentation broth is an alternative to control the pH within fermenter.

Institut für Chemie und Bioanalytik

Listen

Filter

Einstellungen

Sortieren nach

Ergebnisse pro Seite

Ergebnisse nach Hochschule und Institut