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Publikation Augmented feedback system to support physical therapy of non-specific low back pain(Springer, 2010) Brodbeck, Dominique; Degen, Markus; Stanimirov, Michael; Kool, Jan; Scheermesser, Mandy; Oesch, Peter; Neuhaus, Cornelia; Fred, Ana; Filipe, Joaquim; Gamboa, HugoLow back pain is an important problem in industrialized countries. Two key factors limit the effectiveness of physiotherapy: low compliance of patients with repetitive movement exercises, and inadequate awareness of patients of their own posture. The Backtrainer system addresses these problems by real-time monitoring of the spine position, by providing a framework for most common physiotherapy exercises for the low back, and by providing feedback to patients in a motivating way. A minimal sensor configuration was identified as two inertial sensors that measure the orientation of the lower back at two points with three degrees of freedom. The software was designed as a flexible platform to experiment with different hardware, and with various feedback modalities. Basic exercises for two types of movements are provided: mobilizing and stabilizing. We developed visual feedback - abstract as well as in the form of a virtual reality game - and complemented the on-screen graphics with an ambient feedback device. The system was evaluated during five weeks in a rehabilitation clinic with 26 patients and 15 physiotherapists. Subjective satisfaction of subjects was good, and we interpret the results as encouraging indication for the adoption of such a therapy support system by both patients and therapists.04B - Beitrag KonferenzschriftPublikation A method and tool for strategic hospital planning(Springer, 2015) Brodbeck, Dominique; Degen, Markus; Walter, Andreas; Reichlin, Serge; Napierala, Christoph; Fred, Ana; Gamboa, Hugo; Elias, DirkWe developed a visualization tool and a methodology to support strategic planning of hospital service portfolios. Hospitals in Switzerland are reimbursed with a fixed fee per case. The fixed-fee model makes medical services comparable from a financial point of view. In order to take advantage of this model, the data that characterizes the medical services must be operationalized. The method that we developed, centers around a visual metaphor that provides the basis for strategic thinking. It is complemented by a visualization tool that allows visualization, analysis, and modification of service portfolios. Special features enable the tool to be used during live planning sessions. We describe the method, the tool, and its application in strategy workshops for infrastructure planning, reorganization, and resource optimization decisions.04B - Beitrag KonferenzschriftPublikation Dynamics of early skin formation of tiling mortars investigated by microscopy and diffuse reflectance infrared Fourier transformed spectroscopy(Elsevier, 03/2013) Bühler, Theodor; Zurbriggen, Roger; Pieles, Uwe; Huwiler, L.; Raso, RenzoA freshly applied mortar layer is exposed to the environment and forms a skin in the following minutes. Skinning changes significantly the surface properties and therefore limits application procedures. In the case of a tile adhesive, the skin that is formed before the tile is embedded can reduce resulting adhesion properties. Reflected light microscopy reveals that surface “lakes” disappear between 5 and 10 min after mortar application and DRIFTS (diffuse reflectance infrared Fourier transformed spectroscopy) indicates that films of organics and carbonates form simultaneously at the surface.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Comparison of methods for phylogenetic B-cell lineage inference using time-resolved antibody repertoire simulations (AbSim)(Oxford University Press, 31.08.2017) Yermanos, Alexander; Greiff, Victor; Krautler, Nike Julia; Menzel, Ulrike; Dounas, Andreas; Miho, Enkelejda; Oxenius, Annette; Stadler, Tanja; Reddy, Sai T.; Kelso, JanetMotivation: The evolution of antibody repertoires represents a hallmark feature of adaptive B-cell immunity. Recent advancements in high-throughput sequencing have dramatically increased the resolution to which we can measure the molecular diversity of antibody repertoires, thereby offering for the first time the possibility to capture the antigen-driven evolution of B cells. However, there does not exist a repertoire simulation framework yet that enables the comparison of com monly utilized phylogenetic methods with regard to their accuracy in inferring antibody evolution.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Learning the high-dimensional immunogenomic features that predict public and private antibody repertoires(American Association of Immunologists, 15.10.2017) Greiff, Victor; Weber, Cédric R.; Palme, Johannes; Bodenhofer, Ulrich; Miho, Enkelejda; Menzel, Ulrike; Reddy, Sai T.Recent studies have revealed that immune repertoires contain a substantial fraction of public clones, which may be defined as Ab or TCR clonal sequences shared across individuals. It has remained unclear whether public clones possess predictable sequence features that differentiate them from private clones, which are believed to be generated largely stochastically. This knowledge gap represents a lack of insight into the shaping of immune repertoire diversity. Leveraging a machine learning approach capable of capturing the high-dimensional compositional information of each clonal sequence (defined by CDR3), we detected predictive public clone and private clone–specific immunogenomic differences concentrated in CDR3’s N1–D–N2 region, which allowed the prediction of public and private status with 80% accuracy in humans and mice. Our results unexpectedly demonstrate that public, as well as private, clones possess predictable high-dimensional immunogenomic features. Our support vector machine model could be trained effectively on large published datasets (3 million clonal sequences) and was sufficiently robust for public clone prediction across individuals and studies prepared with different library preparation and high-throughput sequencing protocols. In summary, we have uncovered the existence of high-dimensional immunogenomic rules that shape immune repertoire diversity in a predictable fashion. Our approach may pave the way for the construction of a comprehensive atlas of public mouse and human immune repertoires with potential applications in rational vaccine design and immunotherapeutics.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Bioinformatic and statistical analysis of adaptive immune repertoires(Cell Press, 10/2015) Greiff, Victor; Miho, Enkelejda; Menzel, Ulrike; Reddy, Sai T.High-throughput sequencing (HTS) of immune repertoires has enabled the quantitative analysis of adaptive immune responses and offers the potential to revolutionize research in lymphocyte biology, vaccine profiling, and mono clonal antibody engineering. Advances in sequencing technology coupled to an exponential decline in sequencing costs have fueled the recent over whelming interest in immune repertoire sequencing. This, in turn, has sparked the development of numerous methods for bioinformatic and statistics-driven interpretation and visualization of immune repertoires. Here, we review the current literature on bioinformatic and statistical analysis of immune reper toire HTS data and discuss underlying assumptions, applicability, and scope. We further highlight important directions for future research, which could propel immune repertoire HTS to becoming a standard method for measuring adaptive immune responses.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Augmenting adaptive immunity. Progress and challenges in the quantitative engineering and analysis of adaptive immune receptor repertoires(Royal Society of Chemistry, 2019) Brown, Alex J.; Snapkov, Igor; Akbar, Rahmad; Pavlović, Milena; Miho, Enkelejda; Sandve, Geir K.; Greiff, VictorThe adaptive immune system is a natural diagnostic sensor and therapeutic. It recognizes threats earlier than clinical symptoms manifest and neutralizes antigens with exquisite specificity. Recognition specificity and broad reactivity are enabled via adaptive B- and T-cell receptors: the immune receptor repertoire. The human immune system, however, is not omnipotent. Our natural defense system sometimes loses the battle to parasites and microbes and even turns against us in the case of cancer and (autoimmune) inflammatory disease. A long-standing dream of immunoengineers has been, therefore, to mechanistically understand how the immune system “sees”, “reacts” and “remembers” (auto)antigens. Only very recently, experimental and computational methods have achieved sufficient quantitative resolution to start querying and engineering adaptive immunity with high precision. Specifically, these innovations have been applied with the greatest fervency and success in immunotherapy, autoimmunity and vaccine design. The work here highlights advances, challenges and future directions of quantitative approaches which seek to advance the fundamental understanding of immunological phenomena, and reverse engineer the immune system to produce auspicious biopharmaceutical drugs and immunodiagnostics. Our review shows how the merger of fundamental immunology, computational immunology and (digital) biotechnology advances both immunological knowledge and immunoengineering methodologies.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Single-molecule real-time sequencing of the M protein.Toward personalized medicine in monoclonal gammopathies(Wiley, 05.08.2022) Cascino, Pasquale; Nevone, Alice; Piscitelli, Maggie; Scopelliti, Claudia; Girelli, Maria; Mazzini, Giulia; Caminito, Serena; Russo, Giancarlo; Milani, Paolo; Basset, Marco; Foli, Andrea; Fazio, Francesca; Casarini, Simona; Massa, Margherita; Bozzola, Margherita; Ripepi, Jessica; Sesta, Melania Antonietta; Acquafredda, Gloria; De Cicco, Marica; Moretta, Antonia; Rognoni, Paola; Milan, Enrico; Ricagno, Stefano; Lavatelli, Francesca; Petrucci, Maria Teresa; Miho, Enkelejda; Klersy, Catherine; Merlini, Giampaolo; Palladini, Giovanni; Nuvolone, Mario01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Molecular assembly and self‐assembly: molecular nanoscience for future technologies(2003) de Wild, Michael; Berner, Simon; Suzuki, Hitoshi; Ramoino, Luca; Baratoff, Alexis; Jung, Thomas A.In this review the emerging science of single molecules is discussed in the perspective of nanoscale molecular functions and devices. New methods for the controlled assembly of well-defined molecular nanostructures are pre- sented: self assembly and single molecular positioning. The observation and selective modification of conformation, electronics, and molecular mechanics of individual molecules and molecular assemblies by scanning probes is dem- onstrated. To complement this scientific review, some of the possible conse- quences and visions for future developments are discussed, as far as they derive from the presented systems. The prospects of nanoscale science to stim- ulate technological evolution are exemplified.01A - Beitrag in wissenschaftlicher ZeitschriftPublikation Hematocrit measurement - a high precision on-line measurement system based on impedance spectroscopy for use in hemodialysis machines(2009) Trebbels, Dennis; Zengerle, Roland; Hradetzky, DavidThis paper presents a unique technical approach to measure the proportion of blood volume occupied by red blood cells, the hematocrit value (HCT) on-line and in-line. A practical method has been developed to measure without the need for extracting blood samples out of an existing extracorporeal blood circulation system. The new sensor is based on Impedance Spectroscopy and measures electrical properties of the blood at various frequencies. In order to achieve the required precision resolution the sensor geometry has been optimized by Finite Element Analysis. For sensor readout a digital measurement circuitry based on cheap standard components is developed and allows practical implementation of HCT-sensor devices for the first time. Special care has been taken in order to compensate for the temperature effects.04B - Beitrag Konferenzschrift