Hradetzky, David
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Hradetzky, David
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- PublikationExperience with a continuous education program for clinical. regulatory and quality affairs in Northwestern Switzerland(IEEE EMBC, 07/2022) Etter, Philippe; Lucano, Elena; Hradetzky, David [in: 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)]04B - Beitrag Konferenzschrift
- PublikationA new electrospray method for targeted gene delivery(Nature, 03/2018) Boehringer, Stephan; Ruzgys, Paulius; Tamò, Luca; Satkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Hradetzky, David [in: Scientific Reports]A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.01A - Beitrag in wissenschaftlicher Zeitschrift
- PublikationElectrospray application for enchanced delivery of anticancer drugs into cells(Vytautas Magnus university, 2018) Ruzgys, Paulius; Boehringer, Stephan; Tamò, Luca; Šatkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Hradetzky, David [in: The vital nature sign. 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania. Abstract book.]Electrospray is a process based on applied high electric voltage on liquid, leading to the creation and acceleration of small sized droplets. Electrosprayed suspension with exogenous molecules on cells can trigger intracellular delivery. Here, the application study of anticancer drug delivery via electrospray as an antitumor treatment is presented in vitro and in vivo. In vitro experiments were performed on alveolar epithelial cells (A549). Cell viability was performed with flow cytometry assay. Apoptosis/ necrosis was measured using annexin V/PI kit. In vivo experiments were done with C57BL/6J mice. Lewis lung carcinoma (LLC) cell lines were injected intradermally to induce tumor. Electrospray was performed on tumor by cutting the skin. Tumor size was measured with caliper. Results show triggered PI transfer to cells after electrospray process. Moreover, obtained results in vitro showed successful cisplatin and methotrexate intracellular delivery, resulting in around 90 % of cell viability loss with methotrexate and 70 % of cell viability loss with cisplatin. In vivo experiments reveled a tumor decrease by around 2.5 times in 7 days after electrospray treatment at first and third day with both anticancer drugs. Here we show that electrospray method can be applied to local anticancer drug delivery to cells and tissues. Therefore, such method might be adapted as a clinical anticancer therapy in the future.04B - Beitrag Konferenzschrift
- PublikationRapid prototyping method for 3D printed biomaterial constructs with vascular structures(IEEE, 2018) Gullo, Maurizio; Köser, Joachim; Ruckli, Oliver; Eigenmann, Andrej; Hradetzky, David [in: 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)]This paper presents a fabrication method for rapid prototyping of 3D biomaterial constructs with vascular structures. The method relies on poloxamer fugitive ink, which is over casted with a custom-made alginate based model extracellular matrix (ECM). The presented method is simple to implement and compatible with standard cell culture workflows used in biomedical research and pharmaceutical development. We present the material preparation, gelation properties and printing methods in detail. First experiments demonstrate the suitability of the ECM constructs for 3D tissue culture.04B - Beitrag Konferenzschrift
- PublikationMethod for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells(IEEE, 2018) Hradetzky, David; Boehringer, Stephan; Ruzgys, Paulius; Šatkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq [in: 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)]Electrospray is a process based on creation and acceleration of small sized droplets based on electrostatic repulsion. Spraying plasmid containing liquids this process may be used to transfer genes into cells. Within this paper we report on a method for accessing and evaluating the spray modalities using high speed imaging system with a post processing of image data to obtain estimated volume and velocity of emerging droplets first. Second we investigate on the impact of different media on the spray modalities. Third we evaluate the impact of the spray on cell viability and on transfection efficiency of an eGFP plasmid as reporter gene obtained in an in vitro setup on alveolar epithelial like cells (A549).04B - Beitrag Konferenzschrift
- PublikationThe impact of electrospray driven aerosols on the in vitro transfection efficiency for targeted gene and drug delivery(2016) Hradetzky, David; Boehringer, Stephan; Ruzgys, Paulius; Satkauskas, Saulius; Gazdhar, Amiq; Geiser, Thomas06 - Präsentation
- PublikationMonitoring the effectiveness of the vacuum bell during the C-OP-03 treatment of pectus excavatum: technical innovation(2016) Hradetzky, David; Sesia, Sergio; Haecker, Frank-Martin06 - Präsentation
- PublikationThe vacuum bell for conservative treatment of pectus excavatum – the Basel experience(2016) Sesia, Sergio; Hradetzky, David; Haecker, Frank-Martin06 - Präsentation
- PublikationDesign of kidney structures to be cultured under flow conditions in a microfluidic device(2016) Suter-Dick, Laura; Hugot Beaufils, Marina; Chavanne, Philippe; Hradetzky, David06 - Präsentation
- PublikationElectrospray-mediated local chemotherapy of lung carcinoma in mice(European Respiratory Society, 2016) Hradetzky, David; Böhringer, Stephan; Satkauskas, Saulius; Geiser, Thomas; Gazdhar, Amiq; Ruzgys, Paulius [in: European Respiratory Journal]01A - Beitrag in wissenschaftlicher Zeitschrift