2022-07, Etter, Philippe, Lucano, Elena, Hradetzky, David

2018, Ruzgys, Paulius, Boehringer, Stephan, Tamò, Luca, Šatkauskas, Saulius, Geiser, Thomas, Gazdhar, Amiq, Hradetzky, David

Electrospray is a process based on applied high electric voltage on liquid, leading to the creation and acceleration of small sized droplets. Electrosprayed suspension with exogenous molecules on cells can trigger intracellular delivery. Here, the application study of anticancer drug delivery via electrospray as an antitumor treatment is presented in vitro and in vivo. In vitro experiments were performed on alveolar epithelial cells (A549). Cell viability was performed with flow cytometry assay. Apoptosis/ necrosis was measured using annexin V/PI kit. In vivo experiments were done with C57BL/6J mice. Lewis lung carcinoma (LLC) cell lines were injected intradermally to induce tumor. Electrospray was performed on tumor by cutting the skin. Tumor size was measured with caliper. Results show triggered PI transfer to cells after electrospray process. Moreover, obtained results in vitro showed successful cisplatin and methotrexate intracellular delivery, resulting in around 90 % of cell viability loss with methotrexate and 70 % of cell viability loss with cisplatin. In vivo experiments reveled a tumor decrease by around 2.5 times in 7 days after electrospray treatment at first and third day with both anticancer drugs. Here we show that electrospray method can be applied to local anticancer drug delivery to cells and tissues. Therefore, such method might be adapted as a clinical anticancer therapy in the future.

2016, Hradetzky, David, Boehringer, Stephan, Ruzgys, Paulius, Satkauskas, Saulius, Gazdhar, Amiq, Geiser, Thomas

2016, Suter-Dick, Laura, Hugot Beaufils, Marina, Chavanne, Philippe, Hradetzky, David

2021-04-21, Hradetzky, David, Gazdhar, Amiq, Ruzgys, Paulius, Böhringer, Stephan, Sila Dokumaci, Ayse, Hari, Yvonne, Schürch, Christian, Brühl, Frido, Schürch, Stefan, Sönke, Szidat, Riether, Carsten, Satkauskas, Saulius, Geiser, Thomas

An advanced stage, centrally localized invasive tumor is a major cause of sudden death in lung cancer patients. Currently, chemotherapy, radiotherapy, laser ablation, or surgical resection if possible are the available state-of-the-art treatments but none of these guarantee remedy or long-term relief and are often associated with fatal complications. Allowing localized chemotherapy, by direct and confined drug delivery only at the tumor site, could be a promising option for preoperative down staging or palliative therapy. Here we report the localized and targeted application of intra tumor delivery of chemotherapeutics using a novel device based on the principle of electrospray.

2018, Gullo, Maurizio, Köser, Joachim, Ruckli, Oliver, Eigenmann, Andrej, Hradetzky, David

This paper presents a fabrication method for rapid prototyping of 3D biomaterial constructs with vascular structures. The method relies on poloxamer fugitive ink, which is over casted with a custom-made alginate based model extracellular matrix (ECM). The presented method is simple to implement and compatible with standard cell culture workflows used in biomedical research and pharmaceutical development. We present the material preparation, gelation properties and printing methods in detail. First experiments demonstrate the suitability of the ECM constructs for 3D tissue culture.

2016, Hradetzky, David, Sesia, Sergio, Haecker, Frank-Martin

2018-03, Boehringer, Stephan, Ruzgys, Paulius, Tamò, Luca, Satkauskas, Saulius, Geiser, Thomas, Gazdhar, Amiq, Hradetzky, David

A challenge for gene therapy is absence of safe and efficient local delivery of therapeutic genetic material. An efficient and reproducible physical method of electrospray for localized and targeted gene delivery is presented. Electrospray works on the principle of coulombs repulsion, under influence of electric field the liquid carrying genetic material is dispersed into micro droplets and is accelerated towards the targeted tissue, acting as a counter electrode. The accelerated droplets penetrate the targeted cells thus facilitating the transfer of genetic material into the cell. The work described here presents the principle of electrospray for gene delivery, the basic instrument design, and the various optimized parameters to enhance gene transfer in vitro. We estimate a transfection efficiency of up to 60% was achieved. We describe an efficient gene transfer method and a potential electrospray-mediated gene transfer mechanism.

2018, Hradetzky, David, Boehringer, Stephan, Ruzgys, Paulius, Šatkauskas, Saulius, Geiser, Thomas, Gazdhar, Amiq

Electrospray is a process based on creation and acceleration of small sized droplets based on electrostatic repulsion. Spraying plasmid containing liquids this process may be used to transfer genes into cells. Within this paper we report on a method for accessing and evaluating the spray modalities using high speed imaging system with a post processing of image data to obtain estimated volume and velocity of emerging droplets first. Second we investigate on the impact of different media on the spray modalities. Third we evaluate the impact of the spray on cell viability and on transfection efficiency of an eGFP plasmid as reporter gene obtained in an in vitro setup on alveolar epithelial like cells (A549).

2016, Sesia, Sergio, Hradetzky, David, Haecker, Frank-Martin